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Economidis IV, Wagner RP. Studies on the regulation of the branched chain amino acyl-tRNA synthetases of the fungusNeurospora crassa. Wilehm Roux Arch Dev Biol. 1980;189(3):171-180doi: 10.1007/BF00868675.
Economidis, I. V., & Wagner, R. P. (1980). Studies on the regulation of the branched chain amino acyl-tRNA synthetases of the fungusNeurospora crassa. Wilhelm Roux's archives of developmental biology, 189(3), 171-180. https://doi.org/10.1007/BF00868675
Economidis, I V, and Wagner, R P. "Studies on the regulation of the branched chain amino acyl-tRNA synthetases of the fungusNeurospora crassa." Wilhelm Roux's archives of developmental biology vol. 189,3 (1980): 171-180. doi: https://doi.org/10.1007/BF00868675
Economidis IV, Wagner RP. Studies on the regulation of the branched chain amino acyl-tRNA synthetases of the fungusNeurospora crassa. Wilehm Roux Arch Dev Biol. 1980 Oct;189(3):171-180. doi: 10.1007/BF00868675. PMID: 28305172.
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Wilehm Roux Arch Dev Biol. 1980 Oct;189(3):171-180. doi: 10.1007/BF00868675.

Studies on the regulation of the branched chain amino acyl-tRNA synthetases of the fungusNeurospora crassa.

Wilhelm Roux's archives of developmental biology

I V Economidis, R P Wagner

Affiliations

  1. Genetic Institute, University of Texas at Austin, USA.

PMID: 28305172 DOI: 10.1007/BF00868675

Abstract

The specific activities of the branched chain amino acyl-tRNA synthetases from the cytosolic and mitochondrial fractions ofN. crassa were low in dormant conidia and increased during germination, reaching a maximum 8 h after inoculation. This stage of development is characterised by high rates of many other cellular activities.The increases in activity of synthetases of both cytosol and mitochondria are inhibited by cycloheximide indicating that they are synthesized on cytoplasmic ribosomes. The mitochondrial synthetases show a stimulation of their specific activity when mitochondrial RNA and protein synthesis are inhibited by either ethidium bromide or chloramphenicol suggesting that a mitochondrial translation product regulates the synthesis of the mitochondrial synthetases.The activities of amino acyl-tRNA synthetases are dependent on energy production. When respiration is uncoupled from oxidative phosphorylation, synthetase specific activities decrease although the activities of other mitochondrial enzymes like NADH-dehydrogenase increase. This phenomenon suggests that more than one mechanism regulates the synthesis of mitochondrial proteins which are formed on cytoplasmic ribosomes.The synthesis of branched chain amino acyl-tRNA synthetases ofNeurospora is neither repressed by their cognate amino acids, nor is there inhibition by the precursors of these amino acids, as has been observed in other amino acyl-tRNA synthetases of various organism includingNeurospora.

Keywords: Amino acyl-tRNA synthetases; Development; Neurospora; Nuclear-mitochondrial interactions

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