Display options
Cite
Téllez V, Ahumada A, Muro J, et al. Centrifugation of 2-cell mouse ova: cytoplasm stratification and recovery. Rouxs Arch Dev Biol. 1988;197(6):360-365doi: 10.1007/BF00375956.
Téllez, V., Ahumada, A., Muro, J., Sepúlveda, S., & Izquierdo, L. (1988). Centrifugation of 2-cell mouse ova: cytoplasm stratification and recovery. Roux's archives of developmental biology : the official organ of the EDBO, 197(6), 360-365. https://doi.org/10.1007/BF00375956
Téllez, Verónica, et al. "Centrifugation of 2-cell mouse ova: cytoplasm stratification and recovery." Roux's archives of developmental biology : the official organ of the EDBO vol. 197,6 (1988): 360-365. doi: https://doi.org/10.1007/BF00375956
Téllez V, Ahumada A, Muro J, Sepúlveda S, Izquierdo L. Centrifugation of 2-cell mouse ova: cytoplasm stratification and recovery. Rouxs Arch Dev Biol. 1988 Oct;197(6):360-365. doi: 10.1007/BF00375956. PMID: 28305432.
Copy Download .nbib Format: NLM
Share it on

Rouxs Arch Dev Biol. 1988 Oct;197(6):360-365. doi: 10.1007/BF00375956.

Centrifugation of 2-cell mouse ova: cytoplasm stratification and recovery.

Roux's archives of developmental biology : the official organ of the EDBO

Verónica Téllez, Ariel Ahumada, Juan Muro, Soledad Sepúlveda, Luis Izquierdo

Affiliations

  1. Departamento de Biologia, Universidad de Chile, Casilla, 653, Santiago, Chile.

PMID: 28305432 DOI: 10.1007/BF00375956

Abstract

Two-cell mouse ova, which were centrifuged for l h at 70 000-90 000xg, showed a precise stratification of the cytoplasm and an elongation of the nucleus. The ova were fixed at different times and observed by light and electron microscopy using cytochemical methods and detergent extractions. Within 40 min after centrifugation the normal-looking morphology was recovered except for the persisting lipid caps at the centripetal poles of the blastomeres. Cleavage, compaction and blastulation were not prevented by centrifugation. Treatments with colcemid or cytochalasin D delayed but did not impair recovery. These results suggest that a resilient cytoskeletal structure may be involved in this kind of embryonic regulation.

Keywords: Centrifugation; Cytoskeleton; Mouse ova; Regulation

References

  1. Wilehm Roux Arch Dev Biol. 1984 Jul;193(4):205-210 - PubMed
  2. J Cell Biol. 1985 Jan;100(1):333-6 - PubMed
  3. Dev Biol. 1983 Nov;100(1):158-65 - PubMed
  4. Arch Biol Med Exp (Santiago). 1986;19(3-4):279-300 - PubMed
  5. J Embryol Exp Morphol. 1982 Jun;69:115-26 - PubMed
  6. Biol Rev Camb Philos Soc. 1981 Aug;56(3):463-98 - PubMed
  7. Dev Biol. 1986 Mar;114(1):132-40 - PubMed
  8. J Exp Zool. 1972 Apr;180(1):1-12 - PubMed
  9. Dev Biol. 1983 Jan;95(1):227-31 - PubMed
  10. Differentiation. 1980;17(3):161-79 - PubMed
  11. Arch Biol Med Exp (Santiago). 1984 Sep;17(1):29-39 - PubMed
  12. J Embryol Exp Morphol. 1980 Feb;55:211-25 - PubMed
  13. J Embryol Exp Morphol. 1980 Oct;59:89-102 - PubMed
  14. Dev Biol. 1986 Dec;118(2):457-66 - PubMed
  15. Dev Biol. 1983 Nov;100(1):207-13 - PubMed
  16. Dev Biol. 1983 Oct;99(2):447-55 - PubMed

Publication Types