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Moustafa Y, Brugal G. Image analysis of cell proliferation and differentiation in the thymus of the newtPleurodeles waltlii Michah. by SAMBA 200 cell image processing. Wilehm Roux Arch Dev Biol. 1984;193(3):139-148doi: 10.1007/BF00848889.
Moustafa, Y., & Brugal, G. (1984). Image analysis of cell proliferation and differentiation in the thymus of the newtPleurodeles waltlii Michah. by SAMBA 200 cell image processing. Wilhelm Roux's archives of developmental biology, 193(3), 139-148. https://doi.org/10.1007/BF00848889
Moustafa, Yehia, and Brugal, Gérard. "Image analysis of cell proliferation and differentiation in the thymus of the newtPleurodeles waltlii Michah. by SAMBA 200 cell image processing." Wilhelm Roux's archives of developmental biology vol. 193,3 (1984): 139-148. doi: https://doi.org/10.1007/BF00848889
Moustafa Y, Brugal G. Image analysis of cell proliferation and differentiation in the thymus of the newtPleurodeles waltlii Michah. by SAMBA 200 cell image processing. Wilehm Roux Arch Dev Biol. 1984 May;193(3):139-148. doi: 10.1007/BF00848889. PMID: 28305455.
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Wilehm Roux Arch Dev Biol. 1984 May;193(3):139-148. doi: 10.1007/BF00848889.

Image analysis of cell proliferation and differentiation in the thymus of the newtPleurodeles waltlii Michah. by SAMBA 200 cell image processing.

Wilhelm Roux's archives of developmental biology

Yehia Moustafa, Gérard Brugal

Affiliations

  1. Equipe de Microscopie quantitative-CERMO, Université scientifique et médicale de Grenoble, BP no 68, F-38402, Saint-Martin-d'Hères Cedex, France.

PMID: 28305455 DOI: 10.1007/BF00848889

Abstract

The relationships between cell kinetics and nuclear transformations related to cell differentiation were investigated in the thymus of the newt by means of image analysis. A SAMBA 200 cell image processor was used to compute 18 densitometric, textural and morphological parameters on Feulgen-stained thymic nuclei from a few days after hatching of larvae (stage 40) to 1 month after metamorphosis (150 days old). During the first step, cell nuclei were automatically identified as lymphoid or epithelial with a 93.4%-98.7% confidence level when compared with the cytological diagnoses. During the second step, four cell classes were recognized in both epithelial and lymphoid cell populations and assumed to correspond toG

Keywords: Cell kinetics; Differentiation; Image analysis; Proliferation; Thymus

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