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Janeczek J, Born J, Hoppe P, et al. Partial characterization of neural-inducing factors from Xenopus gastrulae Evidence for a larger protein complex containing the factor. Rouxs Arch Dev Biol. 1992;201(1):30-35doi: 10.1007/BF00188773.
Janeczek, J., Born, J., Hoppe, P., & Tiedemann, H. (1992). Partial characterization of neural-inducing factors from Xenopus gastrulae Evidence for a larger protein complex containing the factor. Roux's archives of developmental biology : the official organ of the EDBO, 201(1), 30-35. https://doi.org/10.1007/BF00188773
Janeczek, Jutta, et al. "Partial characterization of neural-inducing factors from Xenopus gastrulae Evidence for a larger protein complex containing the factor." Roux's archives of developmental biology : the official organ of the EDBO vol. 201,1 (1992): 30-35. doi: https://doi.org/10.1007/BF00188773
Janeczek J, Born J, Hoppe P, Tiedemann H. Partial characterization of neural-inducing factors from Xenopus gastrulae Evidence for a larger protein complex containing the factor. Rouxs Arch Dev Biol. 1992 Feb;201(1):30-35. doi: 10.1007/BF00188773. PMID: 28305609.
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Rouxs Arch Dev Biol. 1992 Feb;201(1):30-35. doi: 10.1007/BF00188773.

Partial characterization of neural-inducing factors from Xenopus gastrulae Evidence for a larger protein complex containing the factor.

Roux's archives of developmental biology : the official organ of the EDBO

Jutta Janeczek, Jochen Born, Peter Hoppe, Hildegard Tiedemann

Affiliations

  1. Institut fur Molekularbiologie and Biochemie, Freie Universität Berlin, Arnimallee 22, W-1000, Berlin 33, Germany.

PMID: 28305609 DOI: 10.1007/BF00188773

Abstract

High (Mr ≈ 90-110 kDa) and low (Mr ≈ 15-30 kDa) molecular weight forms of neural-inducing factors have been found in the supernatant of Xenopus gastrula homogenate. The factors, which are protein in nature, have been partially purified by size exclusion high-performance liquid chromatography (HPLC) and sodium dodecyl sulphate (SDS)-polyacrylamide gel electrophoresis. The factor of smaller size, which could be derived from a precursor, is associated with other proteins in a larger complex. The neural-inducing factors are not irreversibly inactivated after chemical deglycosylation with trifluoromethansulfonic acid. The neural-inducing protein which is found in ribonucleoprotein (RNP)-particles was partially purified by hydrophobic chromatography. Possible relationships of the factors in different subcellular fractions and their physiological significance are discussed.

Keywords: Neural induction; Protein factors; Xenopus laevis

References

  1. Cell Differ. 1987 Jul;21(2):131-6 - PubMed
  2. Wilehm Roux Arch Dev Biol. 1983 Jan;192(1):45-47 - PubMed
  3. Anal Biochem. 1975 Dec;69(2):468-73 - PubMed
  4. Proc Natl Acad Sci U S A. 1973 Mar;70(3):778-81 - PubMed
  5. Methods Enzymol. 1977;47:145-9 - PubMed
  6. Wilehm Roux Arch Dev Biol. 1984 Jan;193(1):13-18 - PubMed
  7. Proc Natl Acad Sci U S A. 1980 Dec;77(12):7112-6 - PubMed
  8. Med Biol. 1978 Dec;56(6):361-5 - PubMed
  9. Cell Differ. 1988 Jun;24(1):33-43 - PubMed
  10. J Biol Chem. 1988 Jun 5;263(16):7646-54 - PubMed
  11. Anal Biochem. 1981 Nov 15;118(1):131-7 - PubMed
  12. Roux Arch Dev Biol. 1987 Feb;196(2):137-140 - PubMed
  13. Wilehm Roux Arch Dev Biol. 1979 Sep;187(3):231-244 - PubMed
  14. Proc Natl Acad Sci U S A. 1970 Sep;67(1):164-71 - PubMed
  15. Dev Biol. 1961 Apr;3:140-52 - PubMed
  16. J Biol Chem. 1988 May 5;263(13):6407-15 - PubMed
  17. Prog Clin Biol Res. 1986;226:25-34 - PubMed
  18. J Biol Chem. 1975 May 25;250(10):4007-21 - PubMed
  19. Nature. 1988 Aug 18;334(6183):618-20 - PubMed
  20. Int J Dev Biol. 1989 Mar;33(1):21-48 - PubMed
  21. J Biol Chem. 1947 Dec;171(2):501-5 - PubMed
  22. Anal Biochem. 1976 May 7;72:248-54 - PubMed
  23. Methods Enzymol. 1976;45:678-95 - PubMed
  24. Mol Biol Rep. 1981 Nov 30;8(1):7-10 - PubMed
  25. Methods Enzymol. 1974;34:126-40 - PubMed
  26. Cell Differ Dev. 1989 Jun;27(1):1-7 - PubMed
  27. Methods Enzymol. 1981;80 Pt C:535-61 - PubMed
  28. Roux Arch Dev Biol. 1988 Oct;197(6):339-344 - PubMed
  29. Wilehm Roux Arch Dev Biol. 1984 Jan;193(1):1-12 - PubMed
  30. Methods Enzymol. 1983;91:227-36 - PubMed
  31. Methods Enzymol. 1981;80 Pt C:737-54 - PubMed
  32. Biochemistry. 1967 Jul;6(7):2202-9 - PubMed
  33. Biochem Biophys Res Commun. 1985 Dec 31;133(3):1026-34 - PubMed
  34. Cell Differ. 1986 Sep;19(2):97-101 - PubMed
  35. Eur J Biochem. 1984 Apr 16;140(2):257-64 - PubMed
  36. Biochemistry. 1971 Feb 2;10(3):463-9 - PubMed

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