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Litvin J, King ML. Cell surface proteins of wholeXenopus embryos identified by radioiodination. Rouxs Arch Dev Biol. 1989;198(3):141-147doi: 10.1007/BF02438939.
Litvin, J., & King, M. L. (1989). Cell surface proteins of wholeXenopus embryos identified by radioiodination. Roux's archives of developmental biology : the official organ of the EDBO, 198(3), 141-147. https://doi.org/10.1007/BF02438939
Litvin, Judith, and King, Mary Lou. "Cell surface proteins of wholeXenopus embryos identified by radioiodination." Roux's archives of developmental biology : the official organ of the EDBO vol. 198,3 (1989): 141-147. doi: https://doi.org/10.1007/BF02438939
Litvin J, King ML. Cell surface proteins of wholeXenopus embryos identified by radioiodination. Rouxs Arch Dev Biol. 1989 Oct;198(3):141-147. doi: 10.1007/BF02438939. PMID: 28305716.
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Rouxs Arch Dev Biol. 1989 Oct;198(3):141-147. doi: 10.1007/BF02438939.

Cell surface proteins of wholeXenopus embryos identified by radioiodination.

Roux's archives of developmental biology : the official organ of the EDBO

Judith Litvin, Mary Lou King

Affiliations

  1. Department of Biology, University of Virginia, 22908, Charlottesville, VA, USA.
  2. Department of Cell Biology and Anatomy (R-124), University of Miami, School of Medicine, 33101, Miami, FL, USA.

PMID: 28305716 DOI: 10.1007/BF02438939

Abstract

We have examined the proteins ofXenopus whole oocytes and embryos that are accessible to surface iodination. These cell surface proteins appear to undergo stagespecific modulation during development. The most pronounced changes were observed between oocytes and the 32-cell stage when the number of labelled proteins doubled, and between early and late gastrula when the complexity declined by two thirds. The simplification of the pattern during gastrulation may reflect changes in cell position as endodermal cells move inside leaving ectodermal cells at the external surface. The lectin binding patterns for NP-40 solubilized proteins extracted from oocytes and embryos also changed in a stage-dependent manner. Con A and WGA recognized a complex pattern of glycoproteins containing glucose and mannose residues. In contrast, SAB and RCA binding to galactose residues recognized far fewer glycoproteins. Many of the observed changes occurred during cleavage stages before embryonic gene transcription is initiated and may be due to post-translational modifications.

Keywords: Cell surface; Embryos; Glycoproteins; Xenopus Oocytes

References

  1. Cell. 1982 Oct;30(3):675-86 - PubMed
  2. Nature. 1986 Jul 24-30;322(6077):371-3 - PubMed
  3. Dev Biol. 1984 Nov;106(1):109-20 - PubMed
  4. Cell Differ. 1974 Nov;3(4):193-8 - PubMed
  5. J Morphol. 1972 Feb;136(2):153-79 - PubMed
  6. Cell. 1985 May;41(1):237-47 - PubMed
  7. Dev Biol. 1984 Jul;104(1):117-30 - PubMed
  8. J Cell Sci Suppl. 1986;4:45-70 - PubMed
  9. Biochim Biophys Acta. 1982 Apr 7;686(2):145-52 - PubMed
  10. Anal Biochem. 1985 Oct;150(1):76-85 - PubMed
  11. Annu Rev Biochem. 1983;52:761-99 - PubMed
  12. Nature. 1984 Sep 13-19;311(5982):149-51 - PubMed
  13. J Embryol Exp Morphol. 1983 Oct;77:183-200 - PubMed
  14. Dev Biol. 1987 Feb;119(2):532-9 - PubMed
  15. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 - PubMed
  16. J Embryol Exp Morphol. 1971 Aug;26(1):51-66 - PubMed
  17. Science. 1983 Feb 4;219(4584):450-7 - PubMed
  18. J Exp Zool. 1974 Jun;188(3):361-5 - PubMed
  19. Science. 1987 Oct 23;238(4826):491-7 - PubMed
  20. J Cell Sci. 1986 Jun;82:23-40 - PubMed
  21. J Biol Chem. 1975 May 25;250(10):4007-21 - PubMed
  22. J Embryol Exp Morphol. 1979 Jun;51:137-53 - PubMed
  23. Exp Cell Res. 1976 Aug;101(1):63-70 - PubMed
  24. Cell Motil. 1984;4(2):77-87 - PubMed
  25. Dev Biol. 1985 Oct;111(2):451-63 - PubMed

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