Display options
Cite
Obinata A, Akimoto Y, Hirano H, et al. Short term retinol treatment in vitro induces stable transdifferentiation of chick epidermal cells into mucus-secreting cells. Rouxs Arch Dev Biol. 1991;200(5):289-295doi: 10.1007/BF00241298.
Obinata, A., Akimoto, Y., Hirano, H., & Endo, H. (1991). Short term retinol treatment in vitro induces stable transdifferentiation of chick epidermal cells into mucus-secreting cells. Roux's archives of developmental biology : the official organ of the EDBO, 200(5), 289-295. https://doi.org/10.1007/BF00241298
Obinata, Akiko, et al. "Short term retinol treatment in vitro induces stable transdifferentiation of chick epidermal cells into mucus-secreting cells." Roux's archives of developmental biology : the official organ of the EDBO vol. 200,5 (1991): 289-295. doi: https://doi.org/10.1007/BF00241298
Obinata A, Akimoto Y, Hirano H, Endo H. Short term retinol treatment in vitro induces stable transdifferentiation of chick epidermal cells into mucus-secreting cells. Rouxs Arch Dev Biol. 1991 Nov;200(5):289-295. doi: 10.1007/BF00241298. PMID: 28305798.
Copy Download .nbib Format: NLM
Share it on

Rouxs Arch Dev Biol. 1991 Nov;200(5):289-295. doi: 10.1007/BF00241298.

Short term retinol treatment in vitro induces stable transdifferentiation of chick epidermal cells into mucus-secreting cells.

Roux's archives of developmental biology : the official organ of the EDBO

Akiko Obinata, Yoshihiro Akimoto, Hiroshi Hirano, Hiroyoshi Endo

Affiliations

  1. Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, Teikyo University, 199-0, Sagamiko, Kanagawa, Japan.
  2. Department of Anatomy, Kyorin University School of Medicine, 18, Mitaka, Tokyo, Japan.

PMID: 28305798 DOI: 10.1007/BF00241298

Abstract

Epidermal mucous metaplasia of cultured skin can be induced by treatment with excess retinol for several days (Fell 1957). In the induction of mucous metaplasia, retinol primarily affects the dermal cells and retinol-pretreated dermis can alter epidermal differentiation towards secretory epithelium (Obinata et al. 1987). In this work, we found that mucous metaplasia could be induced by culturing 13-day-old chick embryonic tarsometatarsal skin in medium containing retinol (20 μM) for only 8-24 h, followed by culture in a chemically defined medium (BGJb) without retinol or serum for 6 days. The application of cycloheximide together with retinol during the first 8 h of culture inhibited epidermal mucous metaplasia during subsequent culture for 6 days in BGJb, indicating that induction of a signal(s) in the dermis by excess retinol requires protein synthesis. However, the presence of 20 nM hydrocortisone (Takata et al. 1981) throughout the culture period did not inhibit retinol-induced epidermal mucous metaplasia of the epidermis. This indicates that a brief treatment of the skin with excess retinol determines the direction of epithelial differentiation toward secretory epithelium; this is a simpler in vitro system for the induction of epidermal mucous metaplasia than those established before.

Keywords: Epidermis; Hydrocortisone; Mucous metaplasia; Retinol; Transdifferentiation

References

  1. J Biol Chem. 1985 Feb 10;260(3):1422-6 - PubMed
  2. Proc R Soc Lond B Biol Sci. 1956 Mar 26;146(923):242-56 - PubMed
  3. Exp Cell Res. 1991 Mar;193(1):36-44 - PubMed
  4. Biochim Biophys Acta. 1964 Mar 2;83:1-19 - PubMed
  5. Dev Biol. 1981 Jul 30;85(2):370-9 - PubMed
  6. Biochem J. 1956 Feb;62(2):315-23 - PubMed
  7. Differentiation. 1985;29(1):82-7 - PubMed
  8. J Biol Chem. 1945;161:83-9 - PubMed
  9. J Biol Chem. 1976 Oct 25;251(20):6360-6 - PubMed
  10. J Cell Biol. 1984 Dec;99(6):2279-86 - PubMed
  11. Biochemistry. 1968 Nov;7(11):3821-9 - PubMed
  12. Gen Comp Endocrinol. 1974 Dec;24(4):364-72 - PubMed
  13. Connect Tissue Res. 1978;6(2):101-8 - PubMed
  14. Cell. 1981 Sep;25(3):617-25 - PubMed
  15. Cancer Res. 1976 Mar;36(3):964-72 - PubMed
  16. Anal Biochem. 1977 Jul;81(1):40-6 - PubMed
  17. In Vitro. 1976 Aug;12(8):554-7 - PubMed
  18. Nature. 1987 Dec 3-9;330(6147):444-50 - PubMed
  19. Exp Cell Res. 1961 Oct;25:41-58 - PubMed
  20. J Invest Dermatol. 1977 Nov;69(5):463-76 - PubMed
  21. Exp Cell Res. 1989 May;182(1):33-43 - PubMed
  22. Anal Biochem. 1976 May 7;72:248-54 - PubMed
  23. Exp Cell Res. 1960 Feb;19:99-113 - PubMed
  24. J Biol Chem. 1988 Jun 15;263(17):8390-7 - PubMed
  25. Exp Cell Res. 1973 Jan;76(1):118-26 - PubMed
  26. Dev Biol. 1989 Jun;133(2):322-35 - PubMed
  27. Dev Biol. 1987 Sep;123(1):59-62 - PubMed

Publication Types