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Rouxs Arch Dev Biol. 1987 Mar;196(3):191-201. doi: 10.1007/BF00376314.

The cell biology of Drosophila wing metamorphosis in vitro.

Roux's archives of developmental biology : the official organ of the EDBO

Martin J Milner, Jonathan Muir

Affiliations

  1. Department of Zoology and Marine Biology, University of St. Andrews, KY16 9TS, St. Andrews, Fife, Scotland.

PMID: 28305843 DOI: 10.1007/BF00376314

Abstract

We have examined the metamorphosis of the wing imaginal disc of Drosophila during culture in vitro in the continuous presence of 20-hydroxy ecdysone (0.1 μg/ ml). We find that the sequence of cellular changes in the wing blade during culture closely match those occurring in situ, involving two periods at which the dorsal and ventral surfaces are joined only by cell processes containing trans-alar microtubule arrays. Good pupal and imaginal cuticle secretion is found in this system.

Keywords: Drosophila; Metamorphosis; Tissue culture; Wing imaginal disc

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