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van Loon AE, Goedemans HJ, Weijtens ME, et al. Onset of transcription in Patella vulgata coincides with cell cycle elongation and expression of tubulin genes. Rouxs Arch Dev Biol. 1995;204(3):193-197doi: 10.1007/BF00241272.
van Loon, A. E., Goedemans, H. J., Weijtens, M. E., & Daemen, A. J. (1995). Onset of transcription in Patella vulgata coincides with cell cycle elongation and expression of tubulin genes. Roux's archives of developmental biology : the official organ of the EDBO, 204(3), 193-197. https://doi.org/10.1007/BF00241272
van Loon, André E, et al. "Onset of transcription in Patella vulgata coincides with cell cycle elongation and expression of tubulin genes." Roux's archives of developmental biology : the official organ of the EDBO vol. 204,3 (1995): 193-197. doi: https://doi.org/10.1007/BF00241272
van Loon AE, Goedemans HJ, Weijtens ME, Daemen AJ. Onset of transcription in Patella vulgata coincides with cell cycle elongation and expression of tubulin genes. Rouxs Arch Dev Biol. 1995 Jan;204(3):193-197. doi: 10.1007/BF00241272. PMID: 28305959.
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Rouxs Arch Dev Biol. 1995 Jan;204(3):193-197. doi: 10.1007/BF00241272.

Onset of transcription in Patella vulgata coincides with cell cycle elongation and expression of tubulin genes.

Roux's archives of developmental biology : the official organ of the EDBO

André E van Loon, Hans J Goedemans, Mo E M Weijtens, A J J M Daemen

Affiliations

  1. Department of Experimental Zoology, Utrecht University, Padualaan 8, NL-3584 CH, Utrecht, The Netherlands.

PMID: 28305959 DOI: 10.1007/BF00241272

Abstract

In this study we show that the onset of embryonic transcription in the marine snail Patella vulgata coincides with the start of the sixth cleavage, when the cell-cycle elongates and divisions become asynchronous. Changes in mRNA content before and after onset of transcription were initially demonstrated by in vitro translation of isolated mRNA from different stages. Before the sixth cleavage, three major mRNAs encoding proteins of 36, 50 and 52 kDa were present. These proteins probably correspond to cyclin A and B and ribonucleotide reductase. After this stage, three major proteins with molecular weights of 36.5, 52.5 and 53 kDa were found after in vitro translation. Via hybrid selected translation and differential screening cDNAs corresponding to the 52.5 and 53 kDa proteins were cloned. The encoded proteins resemble tubulins from other animals to a high extent (between 96.5 and 93.1% identity for α-tubulin and 97.9 and 75.9% for β-tubulin). The 36.5 kDa protein is the previously described actin. Both tubulins were expressed at or shortly after the first asynchronous division after the fifth cleavage.

Keywords: Cell cycle; Patella vulgata; Transcription; Tubulin Differentiation

References

  1. Roux Arch Dev Biol. 1993 Mar;202(2):77-84 - PubMed
  2. Biochim Biophys Acta. 1985 Apr 19;824(4):349-56 - PubMed
  3. Cell. 1982 Oct;30(3):675-86 - PubMed
  4. Cell. 1983 Jun;33(2):389-96 - PubMed
  5. EMBO J. 1991 Nov;10(11):3343-9 - PubMed
  6. Dev Suppl. 1991;1:1-10 - PubMed
  7. Mol Cell Biol. 1981 Nov;1(11):983-93 - PubMed
  8. Nature. 1987 Jul 2-8;328(6125):80-2 - PubMed
  9. Cell. 1980 Oct;21(3):717-27 - PubMed
  10. Cell Differ Dev. 1988 Nov;25 Suppl:67-74 - PubMed
  11. Dev Biol. 1989 May;133(1):93-100 - PubMed
  12. Dev Biol. 1982 Nov;94(1):79-86 - PubMed
  13. Dev Biol. 1984 Dec;106(2):289-95 - PubMed
  14. Science. 1989 Nov 3;246(4930):635-40 - PubMed
  15. EMBO J. 1985 Dec 16;4(13A):3463-71 - PubMed
  16. Development. 1990 Dec;110(4):1303-17 - PubMed
  17. Dev Biol. 1978 Mar;63(1):197-212 - PubMed
  18. Science. 1983 Oct 14;222(4620):135-9 - PubMed
  19. Cell. 1986 Mar 28;44(6):871-7 - PubMed
  20. Dev Biol. 1994 Apr;162(2):364-83 - PubMed
  21. Cell. 1980 Mar;19(3):569-77 - PubMed
  22. Development. 1989 Sep;107(1):1-22 - PubMed
  23. Cell. 1987 Feb 13;48(3):399-407 - PubMed
  24. J Morphol. 1977 Oct;154(1):157-86 - PubMed
  25. Dev Biol. 1980 May;76(2):449-64 - PubMed
  26. Cell. 1982 Oct;30(3):687-96 - PubMed
  27. Roux Arch Dev Biol. 1994 Jan;203(4):187-198 - PubMed
  28. Dev Biol. 1980 Sep;79(1):139-48 - PubMed
  29. J Mol Biol. 1983 May 25;166(3):309-27 - PubMed
  30. Cell. 1985 Jun;41(2):349-59 - PubMed
  31. J Cell Biol. 1985 Jun;100(6):1968-76 - PubMed

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