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Sharpe PM, Brunet CL, Foreman DM, et al. Localisation of acidic and basic fibroblast growth factors during mouse palate development and their effects on mouse palate mesenchyme cells in vitro. Rouxs Arch Dev Biol. 1993;202(3):132-143doi: 10.1007/BF00365303.
Sharpe, P. M., Brunet, C. L., Foreman, D. M., & Ferguson, M. W. (1993). Localisation of acidic and basic fibroblast growth factors during mouse palate development and their effects on mouse palate mesenchyme cells in vitro. Roux's archives of developmental biology : the official organ of the EDBO, 202(3), 132-143. https://doi.org/10.1007/BF00365303
Sharpe, P M, et al. "Localisation of acidic and basic fibroblast growth factors during mouse palate development and their effects on mouse palate mesenchyme cells in vitro." Roux's archives of developmental biology : the official organ of the EDBO vol. 202,3 (1993): 132-143. doi: https://doi.org/10.1007/BF00365303
Sharpe PM, Brunet CL, Foreman DM, Ferguson MW. Localisation of acidic and basic fibroblast growth factors during mouse palate development and their effects on mouse palate mesenchyme cells in vitro. Rouxs Arch Dev Biol. 1993 Feb;202(3):132-143. doi: 10.1007/BF00365303. PMID: 28305990.
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Rouxs Arch Dev Biol. 1993 Feb;202(3):132-143. doi: 10.1007/BF00365303.

Localisation of acidic and basic fibroblast growth factors during mouse palate development and their effects on mouse palate mesenchyme cells in vitro.

Roux's archives of developmental biology : the official organ of the EDBO

P M Sharpe, C L Brunet, D M Foreman, M W J Ferguson

Affiliations

  1. Animal and Human Reproduction, Development and Growth Research Group, Department of Cell and Structural Biology, University of Manchester, Stopford Building, Oxford Road, M13 9PT, Manchester, England.

PMID: 28305990 DOI: 10.1007/BF00365303

Abstract

The distribution of acidic and basic fibroblast growth factors (aFGF, bFGF) was mapped during mouse embryonic palate development. Generally, they localised most intensely in the basement membrane and epithelia rather than the mesenchyme. Localisation was predominantly restricted to the palatal nasal, and medial edge epithelia. Staining was particularly intense in the medial edge epithelia at the time of mid-line epithelial seam formation. Intense staining persisted in the epithelia of the degenerating seam and later in the oral and nasal epithelial triangles. Mouse embryonic palate mesenchyme (MEPM) cells cultured in vitro on a variety of substrata (on plastic, on the surface of a collagen gel and within a collagen gel) responded to treatment with aFGF or bFGF. These responses were modulated by the culture substratum. The FGFs stimulated MEPM cell proliferation on plastic and on collagen, but inhibited cell growth in collagen. The FGFs had little effect on protein production when cells were cultured on plastic, but caused a large reduction in on-collagen and incollagen cultures. This reduction was greater in collagenous than non-collagenous proteins. Generally, treatment with FGFs stimulated the production of glycosaminoglycans (GAGs), particularly hyaluronan (HA) and dermatan sulphate (DS). In addition, the size class of HA was shifted to a higher molecular weight form. These data indicate that aFGF and bFGF may play a role in modulating mesenchymal cell matrix biosynthesis, so facilitating palatal epithelial seam degeneration.

Keywords: Extracellular matrix; Growth factors; Palate development; aFGF; bFGF

References

  1. Curr Top Dev Biol. 1990;24:57-93 - PubMed
  2. Differentiation. 1991 Aug;47(3):149-61 - PubMed
  3. Bioessays. 1986 Sep;5(3):129-32 - PubMed
  4. Science. 1989 Aug 18;245(4919):752-5 - PubMed
  5. Development. 1990 Jul;109(3):585-95 - PubMed
  6. J Cell Biol. 1989 Jun;108(6):2459-66 - PubMed
  7. Biochemistry. 1971 Mar 16;10(6):988-94 - PubMed
  8. J Cell Biol. 1987 Dec;105(6 Pt 2):2861-76 - PubMed
  9. Am J Pathol. 1988 Feb;130(2):393-400 - PubMed
  10. Teratology. 1990 Dec;42(6):597-610 - PubMed
  11. Arch Oral Biol. 1987;32(3):163-6 - PubMed
  12. J Cell Biol. 1990 Mar;110(3):753-65 - PubMed
  13. J Cell Sci. 1989 Nov;94 ( Pt 3):577-84 - PubMed
  14. Development. 1988 Apr;102(4):853-67 - PubMed
  15. J Cell Sci. 1979 Aug;38:267-81 - PubMed
  16. Curr Top Dev Biol. 1990;24:261-88 - PubMed
  17. J Cell Sci. 1991 Oct;100 ( Pt 2):371-80 - PubMed
  18. Arch Oral Biol. 1973 Jan;18(1):77-84 - PubMed
  19. Arch Oral Biol. 1992 Jan;37(1):39-48 - PubMed
  20. Dev Biol. 1991 Sep;147(1):207-15 - PubMed
  21. Arch Oral Biol. 1991;36(6):457-71 - PubMed
  22. Development. 1992 Feb;114(2):379-88 - PubMed
  23. Dev Biol. 1990 Oct;141(2):456-60 - PubMed
  24. Development. 1988;103 Suppl:41-60 - PubMed
  25. J Cell Physiol. 1989 Feb;138(2):405-14 - PubMed
  26. Biochem J. 1991 Mar 15;274 ( Pt 3):699-705 - PubMed
  27. J Cell Sci Suppl. 1988;10:195-230 - PubMed
  28. Dev Biol. 1989 Feb;131(2):455-74 - PubMed
  29. Development. 1989 Jan;105(1):131-6 - PubMed
  30. Int J Dev Biol. 1991 Mar;35(1):17-24 - PubMed
  31. Anal Biochem. 1979 Nov 1;99(2):311-5 - PubMed
  32. J Embryol Exp Morphol. 1980 Aug;58:93-106 - PubMed
  33. Development. 1991 Feb;111(2):455-68 - PubMed
  34. J Cell Biol. 1991 Jun;113(5):981-6 - PubMed
  35. J Cell Biol. 1981 Oct;91(1):45-54 - PubMed
  36. Endocr Rev. 1987 May;8(2):95-114 - PubMed
  37. Curr Top Dev Biol. 1984;19:137-64 - PubMed
  38. J Cell Biol. 1982 Oct;95(1):333-9 - PubMed
  39. Proc Natl Acad Sci U S A. 1990 Feb;87(3):1124-8 - PubMed

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