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Gevers P, Coenen AJ, Schipper H, et al. Involvement of fibronectin during epiboly and gastrulation in embryos of the common carp, Cyprinus carpio. Rouxs Arch Dev Biol. 1993;202(3):152-158doi: 10.1007/BF00365305.
Gevers, P., Coenen, A. J., Schipper, H., Stroband, H. W., & Timmermans, L. P. (1993). Involvement of fibronectin during epiboly and gastrulation in embryos of the common carp, Cyprinus carpio. Roux's archives of developmental biology : the official organ of the EDBO, 202(3), 152-158. https://doi.org/10.1007/BF00365305
Gevers, Petra, et al. "Involvement of fibronectin during epiboly and gastrulation in embryos of the common carp, Cyprinus carpio." Roux's archives of developmental biology : the official organ of the EDBO vol. 202,3 (1993): 152-158. doi: https://doi.org/10.1007/BF00365305
Gevers P, Coenen AJ, Schipper H, Stroband HW, Timmermans LP. Involvement of fibronectin during epiboly and gastrulation in embryos of the common carp, Cyprinus carpio. Rouxs Arch Dev Biol. 1993 Feb;202(3):152-158. doi: 10.1007/BF00365305. PMID: 28305992.
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Rouxs Arch Dev Biol. 1993 Feb;202(3):152-158. doi: 10.1007/BF00365305.

Involvement of fibronectin during epiboly and gastrulation in embryos of the common carp, Cyprinus carpio.

Roux's archives of developmental biology : the official organ of the EDBO

Petra Gevers, Anthony J M Coenen, Henk Schipper, Henri W J Stroband, Lucy P M Timmermans

Affiliations

  1. Department of Animal Morphology and Cell Biology, Agricultural University, Marijkeweg 40, P.O. Box 338, 6709 PG, Wageningen, The Netherlands.
  2. Department of Animal Physiology, University of Nijmegen, Toernooiveld, 6525, ED, Nijmegen, The Netherlands.

PMID: 28305992 DOI: 10.1007/BF00365305

Abstract

The present report firstly describes a pilot study in which, during early development of embryos of the common carp, Cyprinus carpio, the cellular adhesion to fibronectin (FN) was blocked by administration of GRGDS peptide (which binds to the FN-receptor). As this treatment resulted in developmental aberrations, suggesting a functional role for FN, the major part of the work was focussed on the distribution of reactivity of anti-FN antibodies during epiboly and gastrulation. GRGDS treatment had a concentration dependent effect on development. Incubation of embryos in 1.5 mg/ml from the 32-cell stage onwards caused a retardation of epiboly, which did not proceed beyond 60%. The embryos did not show involution, as was confirmed by histological study. These preliminary results suggest that FN is involved in both epiboly and gastrulation of carp embryos. During cleavage, no specific extracellular binding of anti-FN antiserum could be observed. However, binding to a number of cell membranes took place from early epiboly onwards. After the onset of gastrulation, we observed a gradually increasing number of the deepest epiblast cells, showing immunostaining on part of their surface, facing the yolk syncytial layer (YSL) or the involuted cells. During early epiboly, anti-FN binding was restricted to areas in front of the migratory hypoblast cells. Later on, binding was found at the border of hypoblast and epiblast cells. At 100% epiboly, some contact areas of epiblast and hypoblast showed a discontinuous lining of reactivity, whilst other areas appeared devoid of anti-FN binding sites. The results indicate that FN is involved in the migration and guidance of hypoblast cells during gastrulation in carp.

Keywords: Fibronectin; Fish development; Gastrulation

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