Display options
Cite
Feder ME, Roberts SP, Bordelon AC. Molecular thermal telemetry of free-ranging adult Drosophila melanogaster. Oecologia. 2000;123(4):460-465doi: 10.1007/s004420000334.
Feder, M. E., Roberts, S. P., & Bordelon, A. C. (2000). Molecular thermal telemetry of free-ranging adult Drosophila melanogaster. Oecologia, 123(4), 460-465. https://doi.org/10.1007/s004420000334
Feder, M E, et al. "Molecular thermal telemetry of free-ranging adult Drosophila melanogaster." Oecologia vol. 123,4 (2000): 460-465. doi: https://doi.org/10.1007/s004420000334
Feder ME, Roberts SP, Bordelon AC. Molecular thermal telemetry of free-ranging adult Drosophila melanogaster. Oecologia. 2000 Jun;123(4):460-465. doi: 10.1007/s004420000334. PMID: 28308753.
Copy Download .nbib Format: NLM
Share it on

Oecologia. 2000 Jun;123(4):460-465. doi: 10.1007/s004420000334.

Molecular thermal telemetry of free-ranging adult Drosophila melanogaster.

Oecologia

M E Feder, S P Roberts, A C Bordelon

Affiliations

  1. Department of Organismal Biology and Anatomy, The University of Chicago, 1027 East 57th Street, Chicago, IL 60637, USA e-mail: [email protected] Tel.: +1-773-7028096, Fax: +1-773-7020037, , , , , , US.
  2. The College, The University of Chicago, 1027 East 57th Street, Chicago, IL 60637, USA, , , , , , US.

PMID: 28308753 DOI: 10.1007/s004420000334

Abstract

The expression of two temperature-sensitive reporter genes, hsp70 and an hsp70-LacZ fusion, in free-ranging adult Drosophila melanogaster indicates that natural thermal stress experienced by such small and mobile insects may be either infrequent or not severe. Levels of the heat-shock protein Hsp70, the major inducible Hsp of Drosophila, were similar in most wild Droso- phila captured after warm days to levels previously reported for unstressed flies in the laboratory. In a transgenic strain transformed with an hsp70-LacZ fusion (i.e., the structural gene encoding bacterial β-galactosidase under control of a heat shock promoter), exposure to temperatures ≥32°C in the laboratory typically resulted in β-galactosidase activities exceeding 140 mOD

Keywords: Key words  Drosophila; Stress; Temperature; Thermal environment; Transgenic

Publication Types