Display options
Cite
Takaku Y, Suzuki H, Kawasaki H, et al. A modified 'NanoSuit®' preserves wet samples in high vacuum: direct observations on cells and tissues in field-emission scanning electron microscopy. R Soc Open Sci. 2017;4(3):160887doi: 10.1098/rsos.160887.
Takaku, Y., Suzuki, H., Kawasaki, H., Ohta, I., Ishii, D., Hirakawa, S., Tsutsui, T., Matsumoto, H., Takehara, S., Nakane, C., Sakaida, K., Suzuki, C., Muranaka, Y., Kikuchi, H., Konno, H., Shimomura, M., & Hariyama, T. (2017). A modified 'NanoSuit®' preserves wet samples in high vacuum: direct observations on cells and tissues in field-emission scanning electron microscopy. Royal Society open science, 4(3), 160887. https://doi.org/10.1098/rsos.160887
Takaku, Yasuharu, et al. "A modified 'NanoSuit®' preserves wet samples in high vacuum: direct observations on cells and tissues in field-emission scanning electron microscopy." Royal Society open science vol. 4,3 (2017): 160887. doi: https://doi.org/10.1098/rsos.160887
Takaku Y, Suzuki H, Kawasaki H, Ohta I, Ishii D, Hirakawa S, Tsutsui T, Matsumoto H, Takehara S, Nakane C, Sakaida K, Suzuki C, Muranaka Y, Kikuchi H, Konno H, Shimomura M, Hariyama T. A modified 'NanoSuit®' preserves wet samples in high vacuum: direct observations on cells and tissues in field-emission scanning electron microscopy. R Soc Open Sci. 2017 Mar 01;4(3):160887. doi: 10.1098/rsos.160887. eCollection 2017 Mar. PMID: 28405375; PMCID: PMC5383832.
Copy Download .nbib Format: NLM
Share it on

R Soc Open Sci. 2017 Mar 01;4(3):160887. doi: 10.1098/rsos.160887. eCollection 2017 Mar.

A modified 'NanoSuit®' preserves wet samples in high vacuum: direct observations on cells and tissues in field-emission scanning electron microscopy.

Royal Society open science

Yasuharu Takaku, Hiroshi Suzuki, Hideya Kawasaki, Isao Ohta, Daisuke Ishii, Satoshi Hirakawa, Takami Tsutsui, Haruko Matsumoto, Sayuri Takehara, Chinatsu Nakane, Kana Sakaida, Chiaki Suzuki, Yoshinori Muranaka, Hirotoshi Kikuchi, Hiroyuki Konno, Masatsugu Shimomura, Takahiko Hariyama

Affiliations

  1. Department of Biology , Hamamatsu University School of Medicine , Higashi-ku, Hamamatsu 431-3192 , Japan.
  2. Department of Chemistry , Hamamatsu University School of Medicine , Higashi-ku, Hamamatsu 431-3192 , Japan.
  3. Department of Regenerative and Infectious Pathology , Hamamatsu University School of Medicine , Higashi-ku, Hamamatsu 431-3192 , Japan.
  4. Laboratory for Ultrastructure Research and Research Equipment Center , Hamamatsu University School of Medicine , Higashi-ku, Hamamatsu 431-3192 , Japan.
  5. Life Science and Applied Chemistry, Graduate School of Engineering , Nagoya Institute of Technology , Gokiso-cho, Showa-ku, Nagoya 466-8555 , Japan.
  6. Department of Dermatology , Hamamatsu University School of Medicine , Higashi-ku, Hamamatsu 431-3192 , Japan.
  7. Second Department of Surgery , Hamamatsu University School of Medicine , Higashi-ku, Hamamatsu 431-3192 , Japan.
  8. Departments of Bio- and Material Photonics , Chitose Institute of Science and Technology , Hokkaido 066-8655 , Japan.

PMID: 28405375 PMCID: PMC5383832 DOI: 10.1098/rsos.160887

Abstract

Although field-emission scanning electron microscopy (FE-SEM) has proven very useful in biomedical research, the high vacuum required (10

Keywords: NanoSuit®; field-emission scanning electron microscope (FE-SEM); high vacuo; living specimen; surface shield effect

References

  1. PLoS One. 2013 Nov 13;8(11):e78563 - PubMed
  2. Microscopy (Oxf). 2014 Aug;63(4):295-300 - PubMed
  3. Proc Biol Sci. 2015 Mar 7;282(1802):null - PubMed
  4. Proc Natl Acad Sci U S A. 2013 May 7;110(19):7631-5 - PubMed
  5. J Microsc. 2002 Dec;208(Pt 3):153-7 - PubMed
  6. Cancer Res. 2007 Nov 1;67(21):10181-9 - PubMed
  7. Nat Commun. 2015 Jun 11;6:7384 - PubMed
  8. Proc Natl Acad Sci U S A. 2004 Mar 9;101(10):3346-51 - PubMed

Publication Types