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Ashshi AM, Alghamdi S, El-Shemi AG, et al. Seroprevalence of Asymptomatic Dengue Virus Infection and Its Antibodies Among Healthy/Eligible Saudi Blood Donors: Findings From Holy Makkah City. Virology (Auckl). 2017;8:1-5doi: 10.1177/1178122X17691261.
Ashshi, A. M., Alghamdi, S., El-Shemi, A. G., Almdani, S., Refaat, B., Mohamed, A. M., Ghazi, H. O., Azhar, E. I., & Al-Allaf, F. A. (2017). Seroprevalence of Asymptomatic Dengue Virus Infection and Its Antibodies Among Healthy/Eligible Saudi Blood Donors: Findings From Holy Makkah City. Virology : research and treatment, 81-5. https://doi.org/10.1177/1178122X17691261
Ashshi, Ahmed M, et al. "Seroprevalence of Asymptomatic Dengue Virus Infection and Its Antibodies Among Healthy/Eligible Saudi Blood Donors: Findings From Holy Makkah City." Virology : research and treatment vol. 8 (2017): 1-5. doi: https://doi.org/10.1177/1178122X17691261
Ashshi AM, Alghamdi S, El-Shemi AG, Almdani S, Refaat B, Mohamed AM, Ghazi HO, Azhar EI, Al-Allaf FA. Seroprevalence of Asymptomatic Dengue Virus Infection and Its Antibodies Among Healthy/Eligible Saudi Blood Donors: Findings From Holy Makkah City. Virology (Auckl). 2017 Feb 24;8:1-5. doi: 10.1177/1178122X17691261. eCollection 2017. PMID: 28469422; PMCID: PMC5348084.
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Virology (Auckl). 2017 Feb 24;8:1-5. doi: 10.1177/1178122X17691261. eCollection 2017.

Seroprevalence of Asymptomatic Dengue Virus Infection and Its Antibodies Among Healthy/Eligible Saudi Blood Donors: Findings From Holy Makkah City.

Virology : research and treatment

Ahmed M Ashshi, Saad Alghamdi, Adel G El-Shemi, Sabir Almdani, Bassem Refaat, Amr M Mohamed, Hani O Ghazi, Esam I Azhar, Faisal A Al-Allaf

Affiliations

  1. Department of Laboratory Medicine, Faculty of Applied Medical Sciences, Umm Al-Qura University, Holy Makkah, Saudi Arabia.
  2. Department of Pharmacology, Faculty of Medicine, Assiut University, Assiut, Egypt.
  3. Immunology Unit, Regional Laboratory of Holy Makkah, Holy Makkah, Saudi Arabia.
  4. Department of Animal Medicine, Faculty of Veterinary Medicine, Assiut University, Assiut, Egypt.
  5. Department of Medical Microbiology, College of Medicine, Umm Al-Qura University, Holy Makkah, Saudi Arabia.
  6. Medical Laboratory Technology Department, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, Saudi Arabia.
  7. Department of Human Genetics, College of Medicine, Umm Al-Qura University, Holy Makkah, Saudi Arabia.

PMID: 28469422 PMCID: PMC5348084 DOI: 10.1177/1178122X17691261

Abstract

BACKGROUND: Threat to blood transfusion-transmitted dengue virus (DENV) and its antibodies has recently emerged worldwide. Dengue fever is an endemic disease in Saudi Arabia, particularly in its Western region. The aim of this study was to estimate the seroprevalence of asymptomatic DENV infection and its antibodies among eligible Saudi blood donors.

METHODS: Serum samples from 910 healthy/eligible adult male Saudi blood donors, who reside in Holy Makkah City of Saudi Arabia, were collected between March 2015 and August 2016 and screened for the detection of DENV nonstructural protein 1 (NS1) antigen and anti-DENV IgM and IgG antibodies using commercial enzyme-linked immunosorbent assay kits (Panbio, Brisbane, QLD, Australia).

RESULTS: Among the tested donors, 48 (5.3%) were seropositive for DENV-NS1 antigen, whereas 50 (5.5%) and 354 (38.9%) were seropositive for anti-DENV IgM and IgG antibodies, respectively. Seropositivity for DENV-NS1 antigen and/or anti-DENV IgM antibody among the tested donors reflects their ongoing asymptomatic viremic infectious stage with DENV during their donation time, whereas high prevalence of anti-DENV IgG seropositivity reflects the high endemicity of dengue disease in this region of Saudi Arabia.

CONCLUSIONS: These results show high prevalence of asymptomatic DENV infection and its antibodies among Saudi blood donors, raising the importance of establishing blood screening for dengue disease at different blood donation services and units in Saudi Arabia to improve the guarantee of blood transfusions and to control DENV dissemination.

Keywords: Saudi Arabia; dengue; healthy/eligible blood donors; seroprevalence

Conflict of interest statement

DECLARATION OF CONFLICTING INTERESTS: The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.

References

  1. Hong Kong Med J. 2008 Jun;14(3):170-7 - PubMed
  2. Transfusion. 2016 May;56(5):1129-37 - PubMed
  3. Emerg Infect Dis. 2013 May;19(5):787-9 - PubMed
  4. Transfus Clin Biol. 2011 Apr;18(2):174-83 - PubMed
  5. Transfus Med. 2009 Apr;19(2):66-77 - PubMed
  6. N Engl J Med. 2010 Jul 29;363(5):484-7 - PubMed
  7. Emerg Infect Dis. 2009 Jan;15(1):8-11 - PubMed
  8. Open Med. 2014 Oct 02;8(4):e105-15 - PubMed
  9. J Infect Dis. 2002 Oct 15;186(8):1165-8 - PubMed
  10. Transfusion. 2011 Aug;51(8):1654-60 - PubMed
  11. J Med Virol. 2010 Aug;82(8):1400-5 - PubMed
  12. Transfusion. 2016 Sep;56(9):2278-85 - PubMed
  13. Lancet. 2007 Nov 10;370(9599):1644-52 - PubMed
  14. Transfusion. 2012 Aug;52(8):1634-9 - PubMed
  15. Rev Bras Hematol Hemoter. 2012;34(3):193-5 - PubMed
  16. PLoS Negl Trop Dis. 2011 Jul;5(7):e1244 - PubMed
  17. Transfusion. 2012 Aug;52(8):1657-66 - PubMed
  18. BMC Infect Dis. 2016 Jul 08;16:320 - PubMed
  19. Front Physiol. 2012 Mar 30;3:70 - PubMed
  20. Transfusion. 2012 Aug;52(8):1667-71 - PubMed
  21. J Infect Dis. 2016 Mar 1;213(5):694-702 - PubMed
  22. Parasit Vectors. 2014 Jun 03;7:258 - PubMed
  23. Virology (Auckl). 2016 Feb 17;7:1-7 - PubMed
  24. Asian J Transfus Sci. 2014 Jan;8(1):64 - PubMed
  25. Blood Transfus. 2015 Jan;13(1):135-8 - PubMed
  26. Transfus Med Hemother. 2014 Feb;41(1):10-7 - PubMed
  27. PLoS One. 2012;7(3):e29957 - PubMed
  28. Virol J. 2015 Jan 16;12:1 - PubMed
  29. Asian J Transfus Sci. 2015 Jul-Dec;9(2):177-80 - PubMed
  30. Transfusion. 2008 Jul;48(7):1355-62 - PubMed
  31. J Virol Methods. 2007 Mar;140(1-2):75-9 - PubMed
  32. J Clin Microbiol. 2002 Feb;40(2):376-81 - PubMed
  33. J Clin Microbiol. 2000 Mar;38(3):1053-7 - PubMed
  34. Biomed Res Int. 2015;2015:834371 - PubMed
  35. World J Virol. 2015 May 12;4(2):113-23 - PubMed
  36. Sci Transl Med. 2011 Dec 21;3(114):114ra128 - PubMed
  37. J Clin Virol. 2004 Nov;31(3):179-84 - PubMed
  38. Emerg Infect Dis. 2014 Mar;20(3):465-8 - PubMed
  39. Science. 2010 May 7;328(5979):745-8 - PubMed
  40. N Engl J Med. 2008 Oct 2;359(14):1526-7 - PubMed
  41. Annu Rev Immunol. 2011;29:587-619 - PubMed
  42. Diagn Microbiol Infect Dis. 2008 Apr;60(4):387-91 - PubMed
  43. PLoS Negl Trop Dis. 2009;3(1):e360 - PubMed

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