Display options
Cite
Jonathan MC, DeMartini J, Van Stigt Thans S, et al. Characterisation of non-degraded oligosaccharides in enzymatically hydrolysed and fermented, dilute ammonia-pretreated corn stover for ethanol production. Biotechnol Biofuels. 2017;10:112doi: 10.1186/s13068-017-0803-3.
Jonathan, M. C., DeMartini, J., Van Stigt Thans, S., Hommes, R., & Kabel, M. A. (2017). Characterisation of non-degraded oligosaccharides in enzymatically hydrolysed and fermented, dilute ammonia-pretreated corn stover for ethanol production. Biotechnology for biofuels, 10112. https://doi.org/10.1186/s13068-017-0803-3
Jonathan, M C, et al. "Characterisation of non-degraded oligosaccharides in enzymatically hydrolysed and fermented, dilute ammonia-pretreated corn stover for ethanol production." Biotechnology for biofuels vol. 10 (2017): 112. doi: https://doi.org/10.1186/s13068-017-0803-3
Jonathan MC, DeMartini J, Van Stigt Thans S, Hommes R, Kabel MA. Characterisation of non-degraded oligosaccharides in enzymatically hydrolysed and fermented, dilute ammonia-pretreated corn stover for ethanol production. Biotechnol Biofuels. 2017 May 02;10:112. doi: 10.1186/s13068-017-0803-3. eCollection 2017. PMID: 28469707; PMCID: PMC5414315.
Copy Download .nbib Format: NLM
Share it on

Biotechnol Biofuels. 2017 May 02;10:112. doi: 10.1186/s13068-017-0803-3. eCollection 2017.

Characterisation of non-degraded oligosaccharides in enzymatically hydrolysed and fermented, dilute ammonia-pretreated corn stover for ethanol production.

Biotechnology for biofuels

M C Jonathan, J DeMartini, S Van Stigt Thans, R Hommes, M A Kabel

Affiliations

  1. Laboratory of Food Chemistry, Wageningen University & Research, Bornse Weilanden 9, 6708 WG Wageningen, The Netherlands.
  2. DuPont Industrial Biosciences (Genencor Division), 925 Page Mill Road, Palo Alto, CA 94304 USA.
  3. DuPont Industrial Biosciences (Genencor International B.V.), Archimedesweg 30, 2333 CN Leiden, The Netherlands.

PMID: 28469707 PMCID: PMC5414315 DOI: 10.1186/s13068-017-0803-3

Abstract

BACKGROUND: Corn stover is lignocellulosic biomass that has potential to be used as raw material for bioethanol production. In the current research, dilute ammonia pretreatment was used to improve the accessibility of corn stover carbohydrates to subsequently added hydrolytic enzymes. Some carbohydrates, however, were still present after enzymatic hydrolysis and fermentation. Hence, this research was aimed to characterise the recalcitrant carbohydrates, especially the oligosaccharides that remained after hydrolysis and fermentation of dilute ammonia-pretreated corn stover (DACS).

RESULTS: About 35% (w/w) of DACS carbohydrates remained after enzymatic hydrolysis and fermentation of the released monosaccharides. One-third of these recalcitrant carbohydrates were water soluble and composed of diverse oligosaccharides. By using UHPLC-MS

CONCLUSIONS: Around one-third of the 35% (w/w) recalcitrant DACS carbohydrates remained as water-soluble saccharides. In this study, more than 50 recalcitrant oligosaccharides were detected, which mostly composed of xylosyl and/or glucosyl residues. The most pronounced oligosaccharides were UAXOS and XGOS. Hence, α-glucuronidase and α-xylosidase were suggested to be added to the enzyme mixture to degrade these oligosaccharides further, and hence the fermentation yield is potentially increased.

Keywords: Aldouronic acid; Corn stover; Glucuronamide; Hexenuronamide; Hexenuronic acid; Xylan; Xyloglucan

References

  1. J Mass Spectrom. 2015 Jan;50(1):247-64 - PubMed
  2. Anal Biochem. 1992 Nov 15;207(1):176-85 - PubMed
  3. Bioresour Technol. 2010 Apr;101(8):2770-81 - PubMed
  4. Nat Protoc. 2012 Sep;7(9):1590-607 - PubMed
  5. Bioresour Technol. 2010 Nov;101(21):8429-38 - PubMed
  6. Curr Opin Plant Biol. 2008 Jun;11(3):301-7 - PubMed
  7. Bioresour Technol. 2005 Dec;96(18):2026-32 - PubMed
  8. Bioresour Technol. 2011 May;102(10):5995-6004 - PubMed
  9. Methods Mol Biol. 2012;893:371-85 - PubMed
  10. Annu Rev Plant Physiol Plant Mol Biol. 1996 Jun;47:445-476 - PubMed
  11. Carbohydr Polym. 2015 Nov 5;132:59-66 - PubMed
  12. Mol Plant. 2009 Sep;2(5):943-65 - PubMed
  13. Analyst. 1994 Jul;119(7):1497-509 - PubMed
  14. Carbohydr Res. 2007 Apr 9;342(5):724-35 - PubMed
  15. FEBS Lett. 2009 May 6;583(9):1457-62 - PubMed
  16. Bioresour Technol. 2005 Dec;96(18):1959-66 - PubMed
  17. J Agric Food Chem. 2010 Nov 10;58(21):11294-301 - PubMed
  18. Biotechnol Biofuels. 2014 May 13;7:72 - PubMed
  19. Biotechnol Biofuels. 2011 Feb 22;4:5 - PubMed
  20. Carbohydr Res. 1998 Jan;306(1-2):265-74 - PubMed
  21. Bioresour Technol. 2012 Apr;110:437-47 - PubMed
  22. Biotechnol Biofuels. 2016 May 18;9:105 - PubMed
  23. Proteomics. 2010 Jun;10(12):2330-6 - PubMed
  24. Carbohydr Res. 2009 Sep 28;344(14):1824-32 - PubMed
  25. Carbohydr Res. 2013 Nov 15;381:33-42 - PubMed
  26. Bioresour Technol. 2005 Apr;96(6):673-86 - PubMed

Publication Types