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Bourgi K, Patel J, Samuel L, et al. Clinical Impact of Nucleic Acid Amplification Testing in the Diagnosis of . Open Forum Infect Dis. 2017;4(2):ofx045doi: 10.1093/ofid/ofx045.
Bourgi, K., Patel, J., Samuel, L., Kieca, A., Johnson, L., & Alangaden, G. (2017). Clinical Impact of Nucleic Acid Amplification Testing in the Diagnosis of . Open forum infectious diseases, 4(2), ofx045. https://doi.org/10.1093/ofid/ofx045
Bourgi, Kassem, et al. "Clinical Impact of Nucleic Acid Amplification Testing in the Diagnosis of ." Open forum infectious diseases vol. 4,2 (2017): ofx045. doi: https://doi.org/10.1093/ofid/ofx045
Bourgi K, Patel J, Samuel L, Kieca A, Johnson L, Alangaden G. Clinical Impact of Nucleic Acid Amplification Testing in the Diagnosis of . Open Forum Infect Dis. 2017 Mar 10;4(2):ofx045. doi: 10.1093/ofid/ofx045. eCollection 2017. PMID: 28470022; PMCID: PMC5407217.
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Open Forum Infect Dis. 2017 Mar 10;4(2):ofx045. doi: 10.1093/ofid/ofx045. eCollection 2017.

Clinical Impact of Nucleic Acid Amplification Testing in the Diagnosis of .

Open forum infectious diseases

Kassem Bourgi, Jaimin Patel, Linoj Samuel, Angela Kieca, Laura Johnson, George Alangaden

Affiliations

  1. Division of Infectious Diseases, Department of Medicine, Vanderbilt University Medical Center, Nashville, Tennessee.
  2. Division of Endocrinology, Department of Medicine, Rowan University School of Osteopathic Medicine, Stratford, New Jersey.
  3. Division of Clinical Microbiology, Department of Pathology and Laboratory Medicine and.
  4. Division of Infectious Diseases, Department of Medicine, Henry Ford Hospital, Detroit, Michigan; and.
  5. Division of Infectious Diseases, Department of Medicine, University of Michigan Health System, Ann Arbor.

PMID: 28470022 PMCID: PMC5407217 DOI: 10.1093/ofid/ofx045

Abstract

BACKGROUND: Nucleic acid amplification (NAA) testing for

METHODS: We identified a retrospective cohort of all patients with AFB smear-positive respiratory specimens at Henry Ford Hospital from January 1, 2001 through December 31, 2011. We examined the association between patients' sociodemographic factors and clinical comorbidities with the likelihood of being diagnosed with MTB. We evaluated the projected change in duration of airborne isolation and unnecessary MTB treatment with introducing NAA testing into clinical decision making for AFB smear-positive patients.

RESULTS: One hundred thirty patients had AFB smear-positive respiratory specimens, 80 of these patients had a positive NAA test result, and 82 patients grew MTB on culture. Nucleic acid amplification testing had a sensitivity and specificity of 97.6% and 100%, respectively. Integrating NAA testing into clinical decision making for patients with AFB-positive smears was associated with a significantly shorter time in airborne isolation (6.0 ± 7.6 vs 23.1 ± 38.0,

CONCLUSIONS: Nucleic acid amplification testing provided a rapid and accurate test in the diagnosis of MTB while significantly reducing the duration of isolation and unnecessary medications in patients with negative NAA test.

© The Author 2017. Published by Oxford University Press on behalf of Infectious Diseases Society of America.

Keywords: Mycobacterium tuberculosis; diagnostic testing; nucleic acid tests.

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