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Petrov PN, Shechtman Y, Moerner WE. Measurement-based estimation of global pupil functions in 3D localization microscopy. Opt Express. 2017;25(7):7945-7959doi: 10.1364/OE.25.007945.
Petrov, P. N., Shechtman, Y., & Moerner, W. E. (2017). Measurement-based estimation of global pupil functions in 3D localization microscopy. Optics express, 25(7), 7945-7959. https://doi.org/10.1364/OE.25.007945
Petrov, Petar N, et al. "Measurement-based estimation of global pupil functions in 3D localization microscopy." Optics express vol. 25,7 (2017): 7945-7959. doi: https://doi.org/10.1364/OE.25.007945
Petrov PN, Shechtman Y, Moerner WE. Measurement-based estimation of global pupil functions in 3D localization microscopy. Opt Express. 2017 Apr 03;25(7):7945-7959. doi: 10.1364/OE.25.007945. PMID: 28380911; PMCID: PMC5810908.
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Opt Express. 2017 Apr 03;25(7):7945-7959. doi: 10.1364/OE.25.007945.

Measurement-based estimation of global pupil functions in 3D localization microscopy.

Optics express

Petar N Petrov, Yoav Shechtman, W E Moerner

PMID: 28380911 PMCID: PMC5810908 DOI: 10.1364/OE.25.007945

Abstract

We report the use of a phase retrieval procedure based on maximum likelihood estimation (MLE) to produce an improved, experimentally calibrated model of a point spread function (PSF) for use in three-dimensional (3D) localization microscopy experiments. The method estimates a global pupil phase function (which includes both the PSF and system aberrations) over the full axial range from a simple calibration scan. The pupil function is used to refine the PSF model and hence enable superior localizations from experimental data. To demonstrate the utility of the procedure, we apply it to experimental data acquired with a microscope employing a tetrapod PSF with a 6 µm axial range. The phase-retrieved model demonstrates significant improvements in both accuracy and precision of 3D localizations relative to the model based on scalar diffraction theory. The localization precision of the phase-retrieved model is shown to be near the limits imposed by estimation theory, and the reproducibility of the procedure is characterized and discussed. Code which performs the phase retrieval algorithm is provided.

References

  1. Nat Methods. 2014 Mar;11(3):267-79 - PubMed
  2. Methods Cell Biol. 2013;114:243-83 - PubMed
  3. Nanoscale. 2011 Nov;3(11):4532-41 - PubMed
  4. Phys Rev Lett. 2014 Sep 26;113(13):133902 - PubMed
  5. Opt Lett. 2014 Jan 15;39(2):275-8 - PubMed
  6. Nano Lett. 2013 Mar 13;13(3):987-93 - PubMed
  7. Nano Lett. 2015 Jun 10;15(6):4194-9 - PubMed
  8. Opt Express. 2012 Feb 27;20(5):4957-67 - PubMed
  9. Nat Photonics. 2016;10 :590-594 - PubMed
  10. Proc SPIE Int Soc Opt Eng. 2010;7571:75710Z - PubMed
  11. Biophys J. 1997 Apr;72(4):1744-53 - PubMed
  12. Nat Photonics. 2014;8:302-306 - PubMed
  13. J Microsc. 2012 Jun;246(3):213-20 - PubMed
  14. Biophys J. 2004 Feb;86(2):1185-200 - PubMed
  15. Opt Express. 2012 Jul 2;20(14):15928-44 - PubMed
  16. Nat Methods. 2010 May;7(5):373-5 - PubMed
  17. J Microsc. 2004 Oct;216(Pt 1):32-48 - PubMed
  18. Biophys J. 2006 Dec 1;91(11):4258-72 - PubMed
  19. Biophys J. 1991 Oct;60(4):910-21 - PubMed
  20. Opt Express. 2013 Dec 2;21(24):29462-87 - PubMed
  21. PLoS One. 2015 Nov 23;10(11):e0142949 - PubMed
  22. Nat Methods. 2006 Oct;3(10):793-5 - PubMed
  23. Proc Natl Acad Sci U S A. 2009 Mar 3;106(9):2995-9 - PubMed
  24. Proc Natl Acad Sci U S A. 2012 Jan 17;109(3):675-9 - PubMed
  25. Science. 2008 Feb 8;319(5864):810-3 - PubMed
  26. Nat Methods. 2010 May;7(5):377-81 - PubMed
  27. PLoS One. 2013;8(1):e53671 - PubMed
  28. Optica. 2015 Nov 20;2(11):985-993 - PubMed
  29. Science. 2006 Sep 15;313(5793):1642-5 - PubMed
  30. Opt Lett. 2011 Jan 15;36(2):202-4 - PubMed

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