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Soufy H, El-Beih NM, Nasr SM, et al. Effect of Egyptian propolis on cryptosporidiosis in immunosuppressed rats with special emphasis on oocysts shedding, leukogram, protein profile and ileum histopathology. Asian Pac J Trop Med. 2017;10(3):253-262doi: 10.1016/j.apjtm.2017.03.004.
Soufy, H., El-Beih, N. M., Nasr, S. M., Abd El-Aziz, T. H., Khalil, F. A. M., Ahmed, Y. F., & Abou Zeina, H. A. A. (2017). Effect of Egyptian propolis on cryptosporidiosis in immunosuppressed rats with special emphasis on oocysts shedding, leukogram, protein profile and ileum histopathology. Asian Pacific journal of tropical medicine, 10(3), 253-262. https://doi.org/10.1016/j.apjtm.2017.03.004
Soufy, Hamdy, et al. "Effect of Egyptian propolis on cryptosporidiosis in immunosuppressed rats with special emphasis on oocysts shedding, leukogram, protein profile and ileum histopathology." Asian Pacific journal of tropical medicine vol. 10,3 (2017): 253-262. doi: https://doi.org/10.1016/j.apjtm.2017.03.004
Soufy H, El-Beih NM, Nasr SM, Abd El-Aziz TH, Khalil FAM, Ahmed YF, Abou Zeina HAA. Effect of Egyptian propolis on cryptosporidiosis in immunosuppressed rats with special emphasis on oocysts shedding, leukogram, protein profile and ileum histopathology. Asian Pac J Trop Med. 2017 Mar;10(3):253-262. doi: 10.1016/j.apjtm.2017.03.004. Epub 2017 Mar 06. PMID: 28442108.
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Asian Pac J Trop Med. 2017 Mar;10(3):253-262. doi: 10.1016/j.apjtm.2017.03.004. Epub 2017 Mar 06.

Effect of Egyptian propolis on cryptosporidiosis in immunosuppressed rats with special emphasis on oocysts shedding, leukogram, protein profile and ileum histopathology.

Asian Pacific journal of tropical medicine

Hamdy Soufy, Nadia M El-Beih, Soad M Nasr, Tamer H Abd El-Aziz, Fathia A M Khalil, Youssef F Ahmed, Hala A A Abou Zeina

Affiliations

  1. Department of Parasitology and Animal Diseases, National Research Centre, 33 Bohouth Street, Dokki, P.O. Box 12622, Giza, Egypt.
  2. Department of Zoology, Faculty of Science, Ain Shams University, Abbassia, P.O. Box 11566, Cairo, Egypt.
  3. Department of Parasitology and Animal Diseases, National Research Centre, 33 Bohouth Street, Dokki, P.O. Box 12622, Giza, Egypt. Electronic address: [email protected].
  4. Department of Reproduction and Artificial Insemination, National Research Centre, 33 Bohouth Street, Dokki, P.O. Box 12622, Giza, Egypt.

PMID: 28442108 DOI: 10.1016/j.apjtm.2017.03.004

Abstract

OBJECTIVES: To investigate the activity of Egyptian propolis extracts (ethanol and water) on cryptosporidiosis in experimentally infected dexamethasone-immunosuppressed rats.

METHODS: A total of 180 male rats (190-220) g BWt were randomly divided into 9 equal groups (G1-G9). Groups of rats were kept as (G1): normal control, (G2-G9): immunosuppressed with dexamethasone and (G3-G9): infected with Cryptosporidium oocysts. Rats from (G4-G9) were given orally ethanol and water extract of propolis (at a dose of 50 mg/kg BWt) and nitazoxanide (standard anti-cryptosporidial drug at a dose of 100 mg/kg BWt) to infected rats with different regimes. Faecal pellets were collected from all groups to monitor oocysts shedding from the 2nd to the 15th day post infection. At the end of the experiment, blood was collected from all groups for determination of leukogram and serum proteins. Ileum specimens were also examined histopathologically.

RESULTS: The highest reduction of oocysts shedding in faecal samples was 88% in rats prophylactically treated with propolis ethanol extract at the 4th dpi, and in rats prophylactically treated with water extract of propolis, was 91% at the 6th dpi. There was a marked increase in neutrophils count and α

CONCLUSIONS: Egyptian propolis extracts have an activity on cryptosporidiosis in rats. Moreover, propolis modulated the immunity in dexamethasone-immunosuppressed rats.

Copyright © 2017 Hainan Medical University. Production and hosting by Elsevier B.V. All rights reserved.

Keywords: Cryptosporidiosis; Histopathology; Leukogram; Propolis; Rat model; Serum proteins

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