Exp Ther Med. 2017 Apr;13(4):1249-1266. doi: 10.3892/etm.2017.4109. Epub 2017 Feb 08.
Anti-climacterium effects of pomegranate concentrated solutions in ovariectomized ddY mice.
Experimental and therapeutic medicine
Su Jin Kang, Beom Rak Choi, Seung Hee Kim, Hae Yeon Yi, Hye Rim Park, Chang Hyun Song, Sae Kwang Ku, Young Joon Lee
Affiliations
Affiliations
- The Medical Research Center for Globalization of Herbal Medicine, Daegu Haany University, Gyeongsan, Gyeongsangbuk-Do 38610, Republic of Korea.
- Department of Preventive Medicine, College of Korean Medicine, Daegu Haany University, Gyeongsan, Gyeongsangbuk-Do 38610, Republic of Korea.
- Research Institute, Health-Love Co., Ltd., Anyang, Kyunggi-Do 13946, Republic of Korea.
- Department of Histology and Anatomy, College of Korean Medicine, Daegu Haany University, Gyeongsan, Gyeongsangbuk-Do 38610, Republic of Korea.
PMID: 28413464
PMCID: PMC5377405 DOI: 10.3892/etm.2017.4109
Abstract
In the present study, the complex anti-climacterium potential of standardized pomegranate concentrated solution (PCS) was investigated using bilateral ovariectomy (OVX) female ddY mice. Changes in body weight and gain during experimental periods, food consumption, serum estradiol levels, total body and abdominal fat densities, abdominal fat pads, and uterus weights were observed, along with the histopathology of abdominal fat pads and uterus for anti-obesity and estrogenic effects. In addition, liver weights, serum aspartate aminotransferase (AST), alanine aminotransferase (ALT) levels, and histopathological inspections were performed to explore the hepato-protective effects. Serum total cholesterol (TC), low density lipoprotein (LDL), high density lipoprotein, and triglyceride (TG) levels were monitored for hypolipidemic effects with total body and femur mean bone mineral density (BMD), right femur wet, dry and ash weights, strength, serum osteocalcin, bone-specific alkaline phosphatase (bALP) contents, and histological and histomorphometrical analyses for anti-osteoporosis activity. As a result of OVX, notable increases in body weight and gains, food consumption, abdominal fat mass densities, weights of abdominal fat pads deposited in the abdominal cavity, and serum AST, ALT, TC, LDL, TG, and osteocalcin levels were observed, along with decreases in the uterus, liver, and femur weights, mean total body and femur BMD, femur strength, serum bALP, and estradiol levels. In addition, marked hypertrophic alterations in adipocytes located in the deposited abdominal fat pads, liver steatosis, uterine disused atrophic changes, and decreases in bone mass and structures of the femur were also observed in OVX control mice with significant increases in bone resorption markers based on histopathological and histomorphometrical analysis. However, these estrogen-deficient climacterium symptoms were significantly (P<0.05 or P<0.01) inhibited after 84 days of continuous treatment with estradiol and PCS (1, 2 and 4 ml/kg), respectively. The present results suggested that PCS was able to effectively inhibit or refine the climacterium symptoms, including obesity, hyperlipidemia, hepatic steatosis, and osteoporosis, induced by OVX in ddY mice.
Keywords: anti-climacterium; ddY mice; ovariectomy; pomegranate concentrated solution
References
- J Steroid Biochem Mol Biol. 2013 Nov;138:100-6 - PubMed
- Malays J Med Sci. 2013 Mar;20(2):25-34 - PubMed
- J Nutr Sci Vitaminol (Tokyo). 2006 Feb;52(1):1-8 - PubMed
- Int J Clin Pract Suppl. 2004 Mar;(139):13-9 - PubMed
- Exp Gerontol. 2004 Sep;39(9):1277-83 - PubMed
- Am J Pathol. 2011 Jul;179(1):240-7 - PubMed
- Clin Nutr. 2008 Feb;27(1):57-64 - PubMed
- Am J Physiol Regul Integr Comp Physiol. 2001 Oct;281(4):R1290-4 - PubMed
- J Biol Chem. 1987 Apr 25;262(12):5592-5 - PubMed
- J Ethnopharmacol. 2007 Dec 3;114(3):400-5 - PubMed
- Exp Ther Med. 2014 Sep;8(3):957-967 - PubMed
- J Food Sci. 2011 Jun-Jul;76(5):C707-13 - PubMed
- Metabolism. 2009 Jan;58(1):38-47 - PubMed
- J Obstet Gynaecol Can. 2002 Jun;24(6):476-84 - PubMed
- Eur J Pharmacol. 2001 Nov 2;430(2-3):243-9 - PubMed
- J Clin Epidemiol. 2001 Feb;54(2):117-20 - PubMed
- Nutrition. 2007 Nov-Dec;23(11-12):853-60 - PubMed
- Zhen Ci Yan Jiu. 2012 Feb;37(1):15-9, 45 - PubMed
- Phytother Res. 2012 Aug;26(8):1182-8 - PubMed
- Nutr Res. 2011 May;31(5):387-96 - PubMed
- Mol Endocrinol. 2003 Oct;17(10):2070-83 - PubMed
- Metabolism. 2011 Oct;60(10):1425-32 - PubMed
- Nutrients. 2015 Apr 09;7(4):2622-47 - PubMed
- World J Gastroenterol. 2004 Jul 1;10(13):1967-70 - PubMed
- Nutr Res. 2012 Nov;32(11):844-55 - PubMed
- Biol Pharm Bull. 2005 Oct;28(10):1879-85 - PubMed
- Best Pract Res Clin Endocrinol Metab. 2004 Sep;18(3):317-32 - PubMed
- Maturitas. 2006 Jan 10;53(1):49-58 - PubMed
- J Nutr Biochem. 2012 Jul;23(7):725-32 - PubMed
- Osteoporos Int. 2004 Sep;15(9):707-15 - PubMed
- Eur J Histochem. 2005 Apr-Jun;49(2):125-30 - PubMed
- J Ethnopharmacol. 2005 May 13;99(1):55-60 - PubMed
- Am J Physiol. 1996 Jan;270(1 Pt 2):R199-206 - PubMed
- J Ethnopharmacol. 2013 Oct 28;150(1):298-307 - PubMed
- Endocr Rev. 1994 Jun;15(3):275-300 - PubMed
- Gynecol Endocrinol. 2005 Apr;20(4):227-35 - PubMed
- Bone. 1998 Nov;23(5):443-51 - PubMed
- Physiol Behav. 2004 Aug;82(1):35-41 - PubMed
- Am J Clin Nutr. 2007 Apr;85(4):1148-56 - PubMed
- Endocrinology. 2011 Jan;152(1):1-3 - PubMed
- Obstet Gynecol. 2002 Mar;99(3):389-94 - PubMed
- Endocrinology. 2011 Jan;152(1):36-47 - PubMed
- Endocrinology. 1998 Mar;139(3):1411-9 - PubMed
- Eur J Nutr. 2014 Aug;53(5):1155-64 - PubMed
- J Ethnopharmacol. 2007 Jan 19;109(2):177-206 - PubMed
- Proc Natl Acad Sci U S A. 2011 Jan 11;108(2):768-73 - PubMed
- Mol Nutr Food Res. 2013 Jun;57(6):1080-8 - PubMed
- Avicenna J Phytomed. 2015 Jan-Feb;5(1):43-55 - PubMed
- Endocr Rev. 1999 Jun;20(3):358-417 - PubMed
- Rev Assoc Med Bras (1992). 2010 Sep-Oct;56(5):558-62 - PubMed
- Int Urogynecol J Pelvic Floor Dysfunct. 2001;12(2):107-10 - PubMed
- Maturitas. 2000 Jan;34 Suppl 1:S3-8 - PubMed
- J Clin Invest. 1998 Apr 15;101(8):1661-9 - PubMed
- Nutrients. 2014 Sep 09;6(9):3536-71 - PubMed
- Diabetes Care. 2007 Mar;30(3):701-6 - PubMed
- Am J Epidemiol. 2001 Sep 15;154(6):489-94 - PubMed
- Environ Health Perspect. 2012 Mar;120(3):332-9 - PubMed
- Arterioscler Thromb Vasc Biol. 2000 Apr;20(4):1150-4 - PubMed
- Endocrinology. 1997 Mar;138(3):863-70 - PubMed
- J Manipulative Physiol Ther. 2002 Jul-Aug;25(6):403-15 - PubMed
- Am J Obstet Gynecol. 1999 Mar;180(3 Pt 2):S312-6 - PubMed
- Lancet. 2005 Apr 30-May 6;365(9470):1543-51 - PubMed
- Arch Womens Ment Health. 2002 Aug;5(1):15-22 - PubMed
- J Med Food. 2015 Jun;18(6):690-7 - PubMed
- Mutat Res. 2010 Dec 21;703(2):99-107 - PubMed
- Endocrinology. 2008 Apr;149(4):1609-17 - PubMed
- J Nutr. 2003 Jun;133(6):1892-7 - PubMed
- Endocrinology. 2007 Dec;148(12):5656-66 - PubMed
- Nutrients. 2013 Oct 30;5(11):4316-32 - PubMed
- Nutrients. 2013 Jul 17;5(7):2734-46 - PubMed
- Rev Assoc Med Bras (1992). 2006 Nov-Dec;52(6):419-23 - PubMed
- Biochem Biophys Res Commun. 1996 Jul 16;224(2):345-50 - PubMed
- Am J Clin Nutr. 1998 Dec;68(6 Suppl):1333S-1346S - PubMed
- Phytochemistry. 2004 Apr;65(8):995-1016 - PubMed
- Obstet Gynecol. 1996 May;87(5 Pt 2):897-904 - PubMed
- Int J Obes Relat Metab Disord. 2002 Aug;26(8):1103-9 - PubMed
- Clin Chem. 1981 Dec;27(12):2002-7 - PubMed
- Clin Biomech (Bristol, Avon). 2002 Nov-Dec;17(9-10):716-8 - PubMed
- Phytother Res. 2007 Feb;21(2):130-4 - PubMed
- J Agric Food Chem. 2002 Jan 2;50(1):81-6 - PubMed
- BMC Complement Altern Med. 2011 Nov 28;11:120 - PubMed
- Ann Intern Med. 1976 Oct;85(4):447-52 - PubMed
- Physiol Behav. 2010 Feb 9;99(2):175-80 - PubMed
- Biol Pharm Bull. 1999 Sep;22(9):920-4 - PubMed
- Philos Trans R Soc Lond B Biol Sci. 2006 Jul 29;361(1471):1251-63 - PubMed
- Nutr Res. 2011 May;31(5):397-403 - PubMed
- Biofactors. 2007;30(3):179-92 - PubMed
- J Ethnopharmacol. 2004 May;92(1):93-101 - PubMed
- J Food Sci. 2013 Aug;78(8):C1166-72 - PubMed
- Cancer Causes Control. 2006 Mar;17(2):169-80 - PubMed
Publication Types