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Jiang B, Wu ZY, Zhu ZC, et al. Expression and role of specificity protein 1 in the sclera remodeling of experimental myopia in guinea pigs. Int J Ophthalmol. 2017;10(4):550-554doi: 10.18240/ijo.2017.04.08.
Jiang, B., Wu, Z. Y., Zhu, Z. C., Ke, G. J., Wen, Y. C., & Sun, S. Q. (2017). Expression and role of specificity protein 1 in the sclera remodeling of experimental myopia in guinea pigs. International journal of ophthalmology, 10(4), 550-554. https://doi.org/10.18240/ijo.2017.04.08
Jiang, Bo, et al. "Expression and role of specificity protein 1 in the sclera remodeling of experimental myopia in guinea pigs." International journal of ophthalmology vol. 10,4 (2017): 550-554. doi: https://doi.org/10.18240/ijo.2017.04.08
Jiang B, Wu ZY, Zhu ZC, Ke GJ, Wen YC, Sun SQ. Expression and role of specificity protein 1 in the sclera remodeling of experimental myopia in guinea pigs. Int J Ophthalmol. 2017 Apr 18;10(4):550-554. doi: 10.18240/ijo.2017.04.08. eCollection 2017. PMID: 28503426; PMCID: PMC5406631.
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Int J Ophthalmol. 2017 Apr 18;10(4):550-554. doi: 10.18240/ijo.2017.04.08. eCollection 2017.

Expression and role of specificity protein 1 in the sclera remodeling of experimental myopia in guinea pigs.

International journal of ophthalmology

Bo Jiang, Zhang-You Wu, Zi-Cheng Zhu, Gen-Jie Ke, Yue-Chun Wen, Si-Qin Sun

Affiliations

  1. Department of Ophthalmology, Anhui Provincial Hospital, Anhui Medical University, Hefei 230001, Anhui Province, China.

PMID: 28503426 PMCID: PMC5406631 DOI: 10.18240/ijo.2017.04.08

Abstract

AIM: To study the expression of collagen I and transcription factor specificity protein 1 (Sp1), a transforming growth factor-β1 (TGF-β1) downstream target, and reveal the impact of the TGF-β1-Sp1 signaling pathway on collagen remodeling in myopic sclera.

METHODS: Seventy-five 1-week-old guinea pigs were randomly divided into normal control, form deprivation myopia (FDM), and self-control groups. FDM was induced for different times using coverage with translucent latex balloons and FDM recovery was performed for 1wk after 4wk treatment; then, changes in refractive power and axial length were measured. Immunohistochemistry and reverse transcription-polymerase chain reaction were used to evaluate dynamic changes in collagen I and Sp1 expression in the sclera of guinea pigs with emmetropia and experimental myopia, and the relationship between collagen I and Sp1 levels was analyzed.

RESULTS: In the FDM group, the refractive power was gradually changed (from 2.09±0.30 D at week 0 to -1.23±0.69 D, -4.17±0.59 D, -7.07±0.56 D, and -4.30±0.58 D at weeks 2, 4, 6, and 1wk after 4wk, respectively;

CONCLUSION: Our data indicate that transcription factor Sp1 may be involved in the regulation of type I collagen synthesis/degradation during myopic sclera remodeling, suggesting that TGF-β1 signaling plays a role in the development and progression of myopia.

Keywords: collagen I; form-deprivation myopia; sclera remodeling; specificity protein 1; transforming growth factor-β1

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