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Trivedi MK, Gangwar M, Mondal SC, et al. Protective effects of tetrahydrocurcumin (THC) on fibroblast and melanoma cell lines in vitro: it's implication for wound healing. J Food Sci Technol. 2017;54(5):1137-1145doi: 10.1007/s13197-017-2525-8.
Trivedi, M. K., Gangwar, M., Mondal, S. C., & Jana, S. (2017). Protective effects of tetrahydrocurcumin (THC) on fibroblast and melanoma cell lines in vitro: it's implication for wound healing. Journal of food science and technology, 54(5), 1137-1145. https://doi.org/10.1007/s13197-017-2525-8
Trivedi, Mahendra Kumar, et al. "Protective effects of tetrahydrocurcumin (THC) on fibroblast and melanoma cell lines in vitro: it's implication for wound healing." Journal of food science and technology vol. 54,5 (2017): 1137-1145. doi: https://doi.org/10.1007/s13197-017-2525-8
Trivedi MK, Gangwar M, Mondal SC, Jana S. Protective effects of tetrahydrocurcumin (THC) on fibroblast and melanoma cell lines in vitro: it's implication for wound healing. J Food Sci Technol. 2017 Apr;54(5):1137-1145. doi: 10.1007/s13197-017-2525-8. Epub 2017 Feb 27. PMID: 28416863; PMCID: PMC5380618.
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J Food Sci Technol. 2017 Apr;54(5):1137-1145. doi: 10.1007/s13197-017-2525-8. Epub 2017 Feb 27.

Protective effects of tetrahydrocurcumin (THC) on fibroblast and melanoma cell lines in vitro: it's implication for wound healing.

Journal of food science and technology

Mahendra Kumar Trivedi, Mayank Gangwar, Sambhu Charan Mondal, Snehasis Jana

Affiliations

  1. Trivedi Global Inc., Henderson, NV 89052 USA.
  2. Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, Madhya Pradesh India.

PMID: 28416863 PMCID: PMC5380618 DOI: 10.1007/s13197-017-2525-8

Abstract

The aim of this study was to investigate the role of tetrahydrocurcumin (THC) against various skin health parameters using in vitro human foreskin fibroblast and melanoma cell lines (i.e. HFF-1 and B16-F10). The study was assessed using cell viability by MTT assay, identification of extracellular matrix component in HFF-1 cell line (i.e. collagen, elastin and hyaluronic acid), melanin synthesis in B16-F10 cells, cell viability against UVB-induced stress in HFF-1 cells, and in vitro wound healing by the scratch assay. THC was found to be safe and nontoxic up to the concentration of 10 µg/mL with improved level of collagen (37.90%), elastin (90.1%), and hyaluronic acid (74.19%) at 1 µg/mL. Besides, melanin was significantly inhibition by 78.5% at the lowest THC concentration of 0.1 µg/mL. UVB-protection rate was significantly improved by 61.2% and improved cell viability by THC in HFF-1 cells, which indicated protection from photoaging. In addition, THC showed significant wound healing activity (78.51%) and greater migration of fibroblast in HFF-1 cells at different time period. It can be concluded from the study that THC can protect the skin matrix with improved extracellular component synthesis and would healing

Keywords: B16-F10; Extracellular matrix; HFF-1; Hyaluronic acid; Scratch assay; Tetrahydrocurcumin

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