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Magadán JG. Radioactive Pulse-Chase Analysis and Immunoprecipitation. Bio Protoc. 2014;4(8)doi: 10.21769/BioProtoc.1101.
Magadán, J. G. (2014). Radioactive Pulse-Chase Analysis and Immunoprecipitation. Bio-protocol, 4(8), . https://doi.org/10.21769/BioProtoc.1101
Magadán, Javier G. "Radioactive Pulse-Chase Analysis and Immunoprecipitation." Bio-protocol vol. 4,8 (2014). doi: https://doi.org/10.21769/BioProtoc.1101
Magadán JG. Radioactive Pulse-Chase Analysis and Immunoprecipitation. Bio Protoc. 2014 Apr 20;4(8). doi: 10.21769/BioProtoc.1101. PMID: 28480315; PMCID: PMC5415304.
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Bio Protoc. 2014 Apr 20;4(8). doi: 10.21769/BioProtoc.1101.

Radioactive Pulse-Chase Analysis and Immunoprecipitation.

Bio-protocol

Javier G Magadán

Affiliations

  1. Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.

PMID: 28480315 PMCID: PMC5415304 DOI: 10.21769/BioProtoc.1101

Abstract

Labeling of newly-synthesized polypeptides with radioactive amino acids followed by immunoprecipitation allows quantitative analysis of the fate of a given protein in a time-dependent manner. This biochemical approach is usually used to study a variety of processes, such as protein folding, co-translational modifications, intracellular transport, and even its rate of degradation. Here, I describe step by step a simple technique to both label newly-synthesized influenza A virus (IAV) hemagglutinin (HA) with [

References

  1. J Virol. 2013 Sep;87(17):9742-53 - PubMed

Publication Types

Grant support