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Kamikawa R, Inagaki Y, Hashimoto T. A novel spliceosome-mediated trans-splicing can change our view on genome complexity of the divergent eukaryote Giardia intestinalis. Biophys Rev. 2011;3(4):193-197doi: 10.1007/s12551-011-0058-3.
Kamikawa, R., Inagaki, Y., & Hashimoto, T. (2011). A novel spliceosome-mediated trans-splicing can change our view on genome complexity of the divergent eukaryote Giardia intestinalis. Biophysical reviews, 3(4), 193-197. https://doi.org/10.1007/s12551-011-0058-3
Kamikawa, Ryoma, et al. "A novel spliceosome-mediated trans-splicing can change our view on genome complexity of the divergent eukaryote Giardia intestinalis." Biophysical reviews vol. 3,4 (2011): 193-197. doi: https://doi.org/10.1007/s12551-011-0058-3
Kamikawa R, Inagaki Y, Hashimoto T. A novel spliceosome-mediated trans-splicing can change our view on genome complexity of the divergent eukaryote Giardia intestinalis. Biophys Rev. 2011 Dec;3(4):193-197. doi: 10.1007/s12551-011-0058-3. Epub 2011 Oct 20. PMID: 28510047; PMCID: PMC5425688.
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Biophys Rev. 2011 Dec;3(4):193-197. doi: 10.1007/s12551-011-0058-3. Epub 2011 Oct 20.

A novel spliceosome-mediated trans-splicing can change our view on genome complexity of the divergent eukaryote Giardia intestinalis.

Biophysical reviews

Ryoma Kamikawa, Yuji Inagaki, Tetsuo Hashimoto

Affiliations

  1. Center for Computational Sciences and Institute of Biological Sciences, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba, Ibaraki, 305-8577, Japan. [email protected].
  2. Center for Computational Sciences and Institute of Biological Sciences, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba, Ibaraki, 305-8577, Japan.

PMID: 28510047 PMCID: PMC5425688 DOI: 10.1007/s12551-011-0058-3

Abstract

Although spliceosomal introns are an abundant landmark in eukaryotic genomes, the nuclear genome of the divergent eukaryote Giardia intestinalis, the causative agent of giardiasis, has been considered as "intron-poor" with only five canonical (cis-spliced) introns. However, three research groups (including ours) have independently reported a novel class of spliceosomal introns in the G. intestinalis genome. Three protein-coding genes are split into pieces in the G. intestinalis genome, and each of the partial coding regions was independently transcribed into polyadenylated premature mRNAs (pre-mRNAs). The two pre-mRNAs directly interact with each other by an intermolecular-stem structure formed between their non-coding portions, and are then processed into mature mRNAs by spliceosome-mediated trans-splicing. Here, we summarize the recently published works on split introns ("splintrons") in the G. intestinalis genome, and then provide our speculation on the functional property of the Giardia spliceosomes based on the putative ratio of splintrons to canonical introns. Finally, we discuss a scenario for the transition from typical GT-AG boundaries to non-typical AT-AC boundaries in a particular splintron of Giardia.

Keywords: Dynein; Giardia intestinalis; Heat shock protein 90; RNA maturation; Spliceosomal introns; Splintrons; Trans-splicing; cis-splicing

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