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Cox G. Biological applications of second harmonic imaging. Biophys Rev. 2011;3(3):131doi: 10.1007/s12551-011-0052-9.
Cox, G. (2011). Biological applications of second harmonic imaging. Biophysical reviews, 3(3), 131. https://doi.org/10.1007/s12551-011-0052-9
Cox, Guy. "Biological applications of second harmonic imaging." Biophysical reviews vol. 3,3 (2011): 131. doi: https://doi.org/10.1007/s12551-011-0052-9
Cox G. Biological applications of second harmonic imaging. Biophys Rev. 2011 Sep;3(3):131. doi: 10.1007/s12551-011-0052-9. Epub 2011 Jul 20. PMID: 28510062; PMCID: PMC5425668.
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Biophys Rev. 2011 Sep;3(3):131. doi: 10.1007/s12551-011-0052-9. Epub 2011 Jul 20.

Biological applications of second harmonic imaging.

Biophysical reviews

Guy Cox

Affiliations

  1. Australian Centre for Microscopy and Microanalysis, University of Sydney, NSW, 2006, Australia. [email protected].

PMID: 28510062 PMCID: PMC5425668 DOI: 10.1007/s12551-011-0052-9

Abstract

Second Harmonic Generation (SHG) microscopy dates back to 1974, but effective biological use of the technique has a history of barely 10 years. It is now widely used to image collagen in many different applications, and is becoming useful for imaging myosin and some polysaccharides. A separate line on research has focussed on SHG dyes, which can provide high-speed indication of membrane potential and are now in use in neurobiology. This review looks at the progress to date in these different fields.

Keywords: Collagen; Myosin; Polysaccharides; SHG; SHG dyes; Second Harmonic Microscopy

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