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Winzor DJ. A historical perspective of the biophysics of the thrombin-heparin system: an example of nonspecific binding and the consequent parking problem in action. Biophys Rev. 2013;5(2):173-185doi: 10.1007/s12551-013-0103-5.
Winzor, D. J. (2013). A historical perspective of the biophysics of the thrombin-heparin system: an example of nonspecific binding and the consequent parking problem in action. Biophysical reviews, 5(2), 173-185. https://doi.org/10.1007/s12551-013-0103-5
Winzor, Donald J. "A historical perspective of the biophysics of the thrombin-heparin system: an example of nonspecific binding and the consequent parking problem in action." Biophysical reviews vol. 5,2 (2013): 173-185. doi: https://doi.org/10.1007/s12551-013-0103-5
Winzor DJ. A historical perspective of the biophysics of the thrombin-heparin system: an example of nonspecific binding and the consequent parking problem in action. Biophys Rev. 2013 Jun;5(2):173-185. doi: 10.1007/s12551-013-0103-5. Epub 2013 Feb 14. PMID: 28510155; PMCID: PMC5425718.
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Biophys Rev. 2013 Jun;5(2):173-185. doi: 10.1007/s12551-013-0103-5. Epub 2013 Feb 14.

A historical perspective of the biophysics of the thrombin-heparin system: an example of nonspecific binding and the consequent parking problem in action.

Biophysical reviews

Donald J Winzor

Affiliations

  1. School of Chemistry and Molecular Biosciences, University of Queensland, Brisbane, Queensland, 4072, Australia. [email protected].

PMID: 28510155 PMCID: PMC5425718 DOI: 10.1007/s12551-013-0103-5

Abstract

Difficulties are encountered in the thermodynamic characterization of interactions between a protein ligand and a linear acceptor, such as a polynucleotide or a polysaccharide, because of the involvement of more than one unit of the polymer chain in each attachment of a protein molecule. Complications arise from the fact that random attachment of ligand to the polymer chain, each unit of which is a potential binding site, initially leads to suboptimal location of protein molecules along the polymer chain-a situation that has to be rectified before the attainment of thermodynamic equilibrium can be realized. Kinetic as well as thermodynamic consequences of such nonspecific binding, termed the parking problem, therefore need to be considered in any quantitative characterization of the interaction between a large ligand and a linear polymer acceptor chain. Results for the thrombin-heparin interaction have been used to illustrate a thermodynamic characterization of nonspecific binding that takes into account these consequences of the parking problem.

Keywords: Nonspecific binding; Parking problem; Protein–polymer interactions; Thrombin–heparin system

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