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Antonov E, Schlembach I, Regestein L, et al. Process relevant screening of cellulolytic organisms for consolidated bioprocessing. Biotechnol Biofuels. 2017;10:106doi: 10.1186/s13068-017-0790-4.
Antonov, E., Schlembach, I., Regestein, L., Rosenbaum, M. A., & Büchs, J. (2017). Process relevant screening of cellulolytic organisms for consolidated bioprocessing. Biotechnology for biofuels, 10106. https://doi.org/10.1186/s13068-017-0790-4
Antonov, Elena, et al. "Process relevant screening of cellulolytic organisms for consolidated bioprocessing." Biotechnology for biofuels vol. 10 (2017): 106. doi: https://doi.org/10.1186/s13068-017-0790-4
Antonov E, Schlembach I, Regestein L, Rosenbaum MA, Büchs J. Process relevant screening of cellulolytic organisms for consolidated bioprocessing. Biotechnol Biofuels. 2017 Apr 24;10:106. doi: 10.1186/s13068-017-0790-4. eCollection 2017. PMID: 28450887; PMCID: PMC5402656.
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Biotechnol Biofuels. 2017 Apr 24;10:106. doi: 10.1186/s13068-017-0790-4. eCollection 2017.

Process relevant screening of cellulolytic organisms for consolidated bioprocessing.

Biotechnology for biofuels

Elena Antonov, Ivan Schlembach, Lars Regestein, Miriam A Rosenbaum, Jochen Büchs

Affiliations

  1. AVT?Biochemical Engineering, RWTH Aachen University, Forckenbeckstr. 51, 52074 Aachen, Germany.
  2. Institute of Applied Microbiology, RWTH Aachen University, Worringerweg 1, 52074 Aachen, Germany.

PMID: 28450887 PMCID: PMC5402656 DOI: 10.1186/s13068-017-0790-4

Abstract

BACKGROUND: Although the biocatalytic conversion of cellulosic biomass could replace fossil oil for the production of various compounds, it is often not economically viable due to the high costs of cellulolytic enzymes. One possibility to reduce costs is consolidated bioprocessing (CBP), integrating cellulase production, hydrolysis of cellulose, and the fermentation of the released sugars to the desired product into one process step. To establish such a process, the most suitable cellulase-producing organism has to be identified. Thereby, it is crucial to evaluate the candidates under target process conditions. In this work, the chosen model process was the conversion of cellulose to the platform chemical itaconic acid by a mixed culture of a cellulolytic fungus with

RESULTS: The screening of five different cellulase producers with the freeze assay identified

CONCLUSIONS: With two complementary methods to quantify cellulase activity and the dynamics of cellulase production for CBP applications,

Keywords: Cellulase activity; Cellulose; Consolidated bioprocessing; Freeze assay; Itaconic acid; Penicillium verruculosum; Respiration activity; Trichoderma reesei

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