Display options
Cite
Tsai CC, Wu PY, Kuo CC, et al. Analysis of microsatellites in the vulnerable orchid Gastrodia flavilabella: the development of microsatellite markers, and cross-species amplification in Gastrodia. Bot Stud. 2014;55(1):72doi: 10.1186/s40529-014-0072-4.
Tsai, C. C., Wu, P. Y., Kuo, C. C., Huang, M. C., Yu, S. K., Hsu, T. W., Chiang, T. Y., & Chiang, Y. C. (2014). Analysis of microsatellites in the vulnerable orchid Gastrodia flavilabella: the development of microsatellite markers, and cross-species amplification in Gastrodia. Botanical studies, 55(1), 72. https://doi.org/10.1186/s40529-014-0072-4
Tsai, Chi-Chu, et al. "Analysis of microsatellites in the vulnerable orchid Gastrodia flavilabella: the development of microsatellite markers, and cross-species amplification in Gastrodia." Botanical studies vol. 55,1 (2014): 72. doi: https://doi.org/10.1186/s40529-014-0072-4
Tsai CC, Wu PY, Kuo CC, Huang MC, Yu SK, Hsu TW, Chiang TY, Chiang YC. Analysis of microsatellites in the vulnerable orchid Gastrodia flavilabella: the development of microsatellite markers, and cross-species amplification in Gastrodia. Bot Stud. 2014 Dec;55(1):72. doi: 10.1186/s40529-014-0072-4. Epub 2014 Oct 09. PMID: 28510952; PMCID: PMC5430336.
Copy Download .nbib Format: NLM
Share it on

Bot Stud. 2014 Dec;55(1):72. doi: 10.1186/s40529-014-0072-4. Epub 2014 Oct 09.

Analysis of microsatellites in the vulnerable orchid Gastrodia flavilabella: the development of microsatellite markers, and cross-species amplification in Gastrodia.

Botanical studies

Chi-Chu Tsai, Pei-Yin Wu, Chia-Chi Kuo, Min-Chun Huang, Sheng-Kun Yu, Tsai-Wen Hsu, Tzen-Yuh Chiang, Yu-Chung Chiang

Affiliations

  1. Crop Improvement Division, Kaohsiung District Agricultural Research and Extension Station, Pingtung, 908, Taiwan.
  2. Department of Life Science, National Cheng Kung University, Tainan, 701, Taiwan.
  3. Department of Nursing, Meiho University, Pingtung, 912, Taiwan.
  4. Department of Biological Sciences, National Sun Yat-sen University, Kaohsiung, 804, Taiwan.
  5. Taiwan Society of Plant Systematics, Kaohsiung, 804, Taiwan.
  6. Endemic Species Research Institute, Nantou, 552, Taiwan. [email protected].
  7. Department of Life Science, National Cheng Kung University, Tainan, 701, Taiwan. [email protected].
  8. Department of Biological Sciences, National Sun Yat-sen University, Kaohsiung, 804, Taiwan. [email protected].

PMID: 28510952 PMCID: PMC5430336 DOI: 10.1186/s40529-014-0072-4

Abstract

BACKGROUND: Gastrodia flabilabella is a mycoheterotrophic orchid that obtains carbohydrates and nutrients from its symbiotic mycorrhizal fungi. The species is an endemic and vulnerable species enlisted in the "A Preliminary Red List of Taiwanese Vascular Plants" according to the IUCN Red List Categories and Criteria Version 3.1. G. flabilabella dwells the underground of broadleaf and coniferous forest with richness litter. Based on herbarium records, this species is distributed in central Taiwan. Twenty eight microsatellite loci were developed in G. flabilabella and were tested for cross-species amplification in additional taxa of G. confusoides, G. elata, and G. javanica. We estimated the genetic variation that is valuable for conservation management and the development of the molecular identification system for G. elata, a traditional Chinese medicine herb.

RESULTS: Microsatellite primer sets were developed from G. flabilabella using the modified AFLP and magnetic bead enrichment method. In total, 257 microsatellite loci were obtained from a magnetic bead enrichment SSR library. Of the 28 microsatellite loci, 16 were polymorphic, in which the number of alleles ranged from 2 to 15, with the observed heterozygosity ranging from 0.02 to 1.00. In total, 15, 13, and 7 of the loci were found to be interspecifically amplifiable to G. confusoides, G. elata, and G. javanica, respectively.

CONCLUSIONS: Amplifiable and transferable microsatellite loci are potentially useful for future studies in investigating intraspecific genetic variation, reconstructing phylogeographic patterns among closely related species, and establishing the standard operating system of molecular identification in Gastrodia.

Keywords: Conservation; Gastrodia; Microsatellites; Mycoheterotrophic orchid; Population genetics; Simple sequence repeat markers

References

  1. Arch Pharm Res. 1999 Apr;22(2):219-24 - PubMed
  2. PLoS One. 2011;6(6):e21298 - PubMed
  3. Am J Bot. 2012 Oct;99(10):e415-20 - PubMed
  4. Theor Appl Genet. 2009 Apr;118(6):1121-31 - PubMed
  5. Nucleic Acids Res. 1999 Jan 15;27(2):573-80 - PubMed
  6. Bioinformatics. 2012 Oct 1;28(19):2537-9 - PubMed
  7. Genet Mol Res. 2014 Feb 19;13(1):986-91 - PubMed
  8. PLoS One. 2012;7(8):e43717 - PubMed
  9. Ann Bot. 2014 Jan;113(2):267-75 - PubMed
  10. BMC Res Notes. 2014 Apr 23;7:255 - PubMed
  11. Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 - PubMed
  12. Am J Bot. 2012 Jun;99(6):e251-4 - PubMed
  13. Mol Ecol. 2002 Jan;11(1):1-16 - PubMed
  14. Molecules. 2014 Apr 22;19(4):5150-62 - PubMed
  15. PLoS One. 2014 Feb 03;9(2):e87469 - PubMed
  16. Genomics. 2011 Aug;98(2):137-44 - PubMed
  17. Int J Mol Sci. 2013 Oct 14;14(10):20414-26 - PubMed
  18. Int J Mol Sci. 2012 Dec 07;13(12):16779-84 - PubMed
  19. Int J Mol Sci. 2012;13(5):6469-91 - PubMed
  20. Yao Xue Xue Bao. 2010 May;45(5):667-72 - PubMed
  21. Mol Ecol Resour. 2010 May;10(3):564-7 - PubMed

Publication Types