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Tu JM, Chang MC, Huang LL, et al. The blue fluorescent protein from Vibrio vulnificus CKM-1 is a useful reporter for plant research. Bot Stud. 2014;55(1):79doi: 10.1186/s40529-014-0079-x.
Tu, J. M., Chang, M. C., Huang, L. L., Chang, C. D., Huang, H. J., Lee, R. H., & Chang, C. C. (2014). The blue fluorescent protein from Vibrio vulnificus CKM-1 is a useful reporter for plant research. Botanical studies, 55(1), 79. https://doi.org/10.1186/s40529-014-0079-x
Tu, Jin-Min, et al. "The blue fluorescent protein from Vibrio vulnificus CKM-1 is a useful reporter for plant research." Botanical studies vol. 55,1 (2014): 79. doi: https://doi.org/10.1186/s40529-014-0079-x
Tu JM, Chang MC, Huang LL, Chang CD, Huang HJ, Lee RH, Chang CC. The blue fluorescent protein from Vibrio vulnificus CKM-1 is a useful reporter for plant research. Bot Stud. 2014 Dec;55(1):79. doi: 10.1186/s40529-014-0079-x. Epub 2014 Dec 17. PMID: 28510958; PMCID: PMC5432841.
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Bot Stud. 2014 Dec;55(1):79. doi: 10.1186/s40529-014-0079-x. Epub 2014 Dec 17.

The blue fluorescent protein from Vibrio vulnificus CKM-1 is a useful reporter for plant research.

Botanical studies

Jin-Min Tu, Ming-Chung Chang, Lynn Lh Huang, Ching-Dong Chang, Hao-Jen Huang, Ruey-Hua Lee, Ching-Chun Chang

Affiliations

  1. Institute of Tropical Plant Sciences, National Cheng Kung University, 1 University Rd, Tainan, 701, Taiwan.
  2. Department of Nutrition, Hung Kuang University, Taichung, 433, Taiwan.
  3. Institute of Biotechnology, National Cheng Kung University, Tainan, 701, Taiwan.
  4. Department of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung, 912, Taiwan.
  5. Department of Life Sciences, National Cheng Kung University, Tainan, 701, Taiwan.
  6. Institute of Tropical Plant Sciences, National Cheng Kung University, 1 University Rd, Tainan, 701, Taiwan. [email protected].
  7. Institute of Biotechnology, National Cheng Kung University, Tainan, 701, Taiwan. [email protected].

PMID: 28510958 PMCID: PMC5432841 DOI: 10.1186/s40529-014-0079-x

Abstract

BACKGROUND: The mBFP is an improved variant of NADPH-dependent blue fluorescent protein that was originally identified from the non-bioluminescent pathogenic bacteria Vibrio vulnificus CKM-1. To explore the application of mBFP in plants, the mBFP gene expression was driven by one of the three promoters, namely, leaf-specific (RbcS), hypoxia-inducible (Adh) or auxin-inducible (DR5) promoters, in different plant tissues such as leaves, roots and flowers under diverse treatments. In addition, the expressed mBFP protein was targeted to five subcellular compartments such as cytosol, endoplasmic reticulum, apoplast, chloroplast and mitochondria, respectively, in plant cells.

RESULTS: When the mBFP was transiently expressed in the tobacco leaves and floral tissues of moth orchid, the cytosol and apoplast exhibited brighter blue fluorescence than other compartments. The recombinant mBFP-mS

CONCLUSION: The NADPH-dependent blue fluorescent protein could serve as a useful reporter in plants under aerobic or hypoxic condition. However, to avoid masking the mitochondrial targeting signal, fusing mBFP as a fusion tag in the C-terminal will be better when the mBFP is applied in mitochondria trafficking study. Furthermore, mBFP might have the potential to be further adopted as a NADPH biosensor in plant cells. Future codon optimization of mBFP for plants could significantly enhance its brightness and expand its potential applications.

Keywords: Blue fluorescence protein; NADPH; Reporter; Vibrio vulnificus CKM-1

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