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Hua S, Chen ZH, Zhang Y, et al. Chlorophyll and carbohydrate metabolism in developing silique and seed are prerequisite to seed oil content of Brassica napus L. Bot Stud. 2014;55(1):34doi: 10.1186/1999-3110-55-34.
Hua, S., Chen, Z. H., Zhang, Y., Yu, H., Lin, B., & Zhang, D. (2014). Chlorophyll and carbohydrate metabolism in developing silique and seed are prerequisite to seed oil content of Brassica napus L. Botanical studies, 55(1), 34. https://doi.org/10.1186/1999-3110-55-34
Hua, Shuijin, et al. "Chlorophyll and carbohydrate metabolism in developing silique and seed are prerequisite to seed oil content of Brassica napus L." Botanical studies vol. 55,1 (2014): 34. doi: https://doi.org/10.1186/1999-3110-55-34
Hua S, Chen ZH, Zhang Y, Yu H, Lin B, Zhang D. Chlorophyll and carbohydrate metabolism in developing silique and seed are prerequisite to seed oil content of Brassica napus L. Bot Stud. 2014 Dec;55(1):34. doi: 10.1186/1999-3110-55-34. Epub 2014 Mar 19. PMID: 28510961; PMCID: PMC5432831.
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Bot Stud. 2014 Dec;55(1):34. doi: 10.1186/1999-3110-55-34. Epub 2014 Mar 19.

Chlorophyll and carbohydrate metabolism in developing silique and seed are prerequisite to seed oil content of Brassica napus L.

Botanical studies

Shuijin Hua, Zhong-Hua Chen, Yaofeng Zhang, Huasheng Yu, Baogang Lin, Dongqing Zhang

Affiliations

  1. Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, P.R. China. [email protected].
  2. School of Science and Health, University of Western Sydney, Penrith, 2751NSW, Australia.
  3. Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, P.R. China.
  4. Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, P.R. China. [email protected].

PMID: 28510961 PMCID: PMC5432831 DOI: 10.1186/1999-3110-55-34

Abstract

BACKGROUND: Although the seed oil content in canola is a crucial quality determining trait, the regulatory mechanisms of its formation are not fully discovered. This study compared the silique and seed physiological characteristics including fresh and dry weight, seed oil content, chlorophyll content, and carbohydrate content in a high oil content line (HOCL) and a low oil content line (LOCL) of canola derived from a recombinant inbred line in 2010, 2011, and 2012. The aim of the investigation is to uncover the physiological regulation of silique and seed developmental events on seed oil content in canola.

RESULTS: On average, 83% and 86% of silique matter while 69% and 63% of seed matter was produced before 30 days after anthesis (DAA) in HOCL and LOCL, respectively, over three years. Furthermore, HOCL exhibited significantly higher fresh and dry matter at most developmental stages of siliques and seeds. From 20 DAA, lipids were deposited in the seed of HOCL significantly faster than that of LOCL, which was validated by transmission electron microscopy, showing that HOCL accumulates considerable more oil bodies in the seed cells. Markedly higher silique chlorophyll content was observed in HOCL consistently over the three consecutive years, implying a higher potential of photosynthetic capacity in siliques of HOCL. As a consequence, HOCL exhibited significantly higher content of fructose, glucose, sucrose, and starch mainly at 20 to 45 DAA, a key stage of seed lipid deposition. Moreover, seed sugar content was usually higher than silique indicating the importance of sugar transportation from siliques to seeds as substrate for lipid biosynthesis. The much lower silique cellulose content in HOCL was beneficial for lipid synthesis rather than consuming excessive carbohydrate for cell wall.

CONCLUSIONS: Superior physiological characteristics of siliques in HOCL showed advantage to produce more photosynthetic assimilates, which were highly correlated to seed oil contents.

Keywords: Biomass; Brassica napus L; Carbohydrate; Chlorophyll content; Seed oil content

References

  1. Plant Cell Physiol. 2012 Aug;53(8):1380-90 - PubMed
  2. J Integr Plant Biol. 2009 Aug;51(8):792-9 - PubMed
  3. Plant Physiol. 2012 Oct;160(2):1023-36 - PubMed
  4. Plant Physiol. 2005 Oct;139(2):701-12 - PubMed
  5. Planta. 2007 Aug;226(3):773-83 - PubMed
  6. Plant Physiol Biochem. 2009 Jun;47(6):448-55 - PubMed
  7. J Exp Bot. 2008;59(15):4247-57 - PubMed
  8. New Phytol. 2010 Aug;187(3):791-804 - PubMed
  9. Plant J. 2009 Feb;57(4):690-705 - PubMed
  10. Phytochemistry. 2011 Jan;72(1):59-67 - PubMed
  11. Plant J. 2012 Feb;69(3):432-44 - PubMed
  12. Plant Physiol. 2008 Sep;148(1):504-18 - PubMed
  13. Plant Cell. 2007 Jun;19(6):2006-22 - PubMed
  14. Plant J. 2006 Apr;46(1):69-84 - PubMed
  15. Plant Physiol. 1997 May;114(1):153-160 - PubMed
  16. Plant Physiol. 2012 Jul;159(3):1221-34 - PubMed
  17. New Phytol. 2011 Jun;190(4):838-53 - PubMed
  18. J Exp Bot. 2003 Jan;54(382):477-88 - PubMed
  19. Plant Physiol. 2008 Jan;146(1):277-88 - PubMed
  20. Plant Physiol. 1998 Sep;118(1):91-101 - PubMed
  21. Plant Cell. 1995 Sep;7(9):1395-403 - PubMed
  22. J Exp Bot. 2003 Jan;54(381):259-70 - PubMed
  23. Plant Physiol. 2004 Sep;136(1):2676-86 - PubMed
  24. J Exp Bot. 2001 Aug;52(361):1655-63 - PubMed
  25. Theor Appl Genet. 2011 Jan;122(1):21-31 - PubMed
  26. Plant Physiol. 2011 Jul;156(3):1577-88 - PubMed
  27. Plant Physiol. 2004 Oct;136(2):3341-9 - PubMed
  28. Theor Appl Genet. 2012 Jul;125(2):285-96 - PubMed
  29. J Exp Bot. 2003 Aug;54(389):1919-30 - PubMed
  30. Plant Physiol. 2006 Sep;142(1):88-97 - PubMed
  31. Theor Appl Genet. 2006 Nov;113(7):1331-45 - PubMed
  32. Plant Cell. 2002 Jun;14(6):1191-206 - PubMed
  33. Photosynth Res. 1981 Jun;2(2):115-41 - PubMed
  34. Plant Physiol. 2000 Dec;124(4):1582-94 - PubMed
  35. Plant Physiol. 2010 Apr;152(4):2078-87 - PubMed
  36. BMC Genomics. 2009 Jun 02;10:256 - PubMed
  37. Plant Physiol. 2009 May;150(1):27-41 - PubMed
  38. Plant Physiol. 2003 Jan;131(1):228-36 - PubMed
  39. Plant Physiol. 2006 May;141(1):32-46 - PubMed
  40. J Exp Bot. 2008;59(12):3283-95 - PubMed
  41. Anal Biochem. 1969 Dec;32(3):420-4 - PubMed
  42. Plant Physiol. 2008 Aug;147(4):2121-30 - PubMed
  43. J Exp Bot. 2000 Dec;51(353):1991-9 - PubMed
  44. J Exp Bot. 2003 Jan;54(382):457-65 - PubMed
  45. Plant Physiol. 2009 Oct;151(2):857-68 - PubMed
  46. Plant Physiol. 1992 Jun;99(2):700-6 - PubMed
  47. J Exp Bot. 2010 Oct;61(15):4313-24 - PubMed
  48. Plant Physiol. 2008 Oct;148(2):1042-54 - PubMed

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