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Zhou C, Zhang J, Zhao SJ, et al. An active Catharanthus roseus desacetoxyvindoline-4-hydroxylase-like gene and its transcriptional regulatory profile. Bot Stud. 2014;55(1):29doi: 10.1186/1999-3110-55-29.
Zhou, C., Zhang, J., Zhao, S. J., & Hu, Z. B. (2014). An active Catharanthus roseus desacetoxyvindoline-4-hydroxylase-like gene and its transcriptional regulatory profile. Botanical studies, 55(1), 29. https://doi.org/10.1186/1999-3110-55-29
Zhou, Chen, et al. "An active Catharanthus roseus desacetoxyvindoline-4-hydroxylase-like gene and its transcriptional regulatory profile." Botanical studies vol. 55,1 (2014): 29. doi: https://doi.org/10.1186/1999-3110-55-29
Zhou C, Zhang J, Zhao SJ, Hu ZB. An active Catharanthus roseus desacetoxyvindoline-4-hydroxylase-like gene and its transcriptional regulatory profile. Bot Stud. 2014 Dec;55(1):29. doi: 10.1186/1999-3110-55-29. Epub 2014 Mar 13. PMID: 28510984; PMCID: PMC5430314.
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Bot Stud. 2014 Dec;55(1):29. doi: 10.1186/1999-3110-55-29. Epub 2014 Mar 13.

An active Catharanthus roseus desacetoxyvindoline-4-hydroxylase-like gene and its transcriptional regulatory profile.

Botanical studies

Chen Zhou, Jin Zhang, Shu-Juan Zhao, Zhi-Bi Hu

Affiliations

  1. The SATCM Key Laboratory for New Resources & Quality Evaluation of Chinese Medicines and Shanghai Key Laboratory of Complex Prescription, Institute of Traditional Chinese Material Medica, Shanghai University of Traditional Chinese Medicine, 201203, Shanghai, China.
  2. The SATCM Key Laboratory for New Resources & Quality Evaluation of Chinese Medicines and Shanghai Key Laboratory of Complex Prescription, Institute of Traditional Chinese Material Medica, Shanghai University of Traditional Chinese Medicine, 201203, Shanghai, China. [email protected].
  3. The SATCM Key Laboratory for New Resources & Quality Evaluation of Chinese Medicines and Shanghai Key Laboratory of Complex Prescription, Institute of Traditional Chinese Material Medica, Shanghai University of Traditional Chinese Medicine, 201203, Shanghai, China. [email protected].

PMID: 28510984 PMCID: PMC5430314 DOI: 10.1186/1999-3110-55-29

Abstract

BACKGROUND: Desacetoxyvindoline-4-hydroxylase is a key enzyme in the biosynthesis of vindoline, the important intermediate leading to vinblastine and vincristine in Catharanthus roseus.

RESULTS: A d4h-like gene has been isolated from C. roseus C

CONCLUSION: These results suggest that d4h-like is closely related with d4h in the genetic evolution but with different transcriptional expression profiles. It may be revolved in the hormone-independency of C

Keywords: C20hi cells; Catharanthus roseus; Desacetoxyvindoline-4-hydroxylase; GUS activity; Real-time quantitative PCR; Transient expression; d4h-like

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