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Lee MTW, Maruani A, Richards DA, et al. Enabling the controlled assembly of antibody conjugates with a loading of two modules without antibody engineering. Chem Sci. 2016;8(3):2056-2060doi: 10.1039/c6sc03655d.
Lee, M. T. W., Maruani, A., Richards, D. A., Baker, J. R., Caddick, S., & Chudasama, V. (2017). Enabling the controlled assembly of antibody conjugates with a loading of two modules without antibody engineering. Chemical science, 8(3), 2056-2060. https://doi.org/10.1039/c6sc03655d
Lee, Maximillian T W, et al. "Enabling the controlled assembly of antibody conjugates with a loading of two modules without antibody engineering." Chemical science vol. 8,3 (2017): 2056-2060. doi: https://doi.org/10.1039/c6sc03655d
Lee MTW, Maruani A, Richards DA, Baker JR, Caddick S, Chudasama V. Enabling the controlled assembly of antibody conjugates with a loading of two modules without antibody engineering. Chem Sci. 2017 Mar 01;8(3):2056-2060. doi: 10.1039/c6sc03655d. Epub 2016 Nov 28. PMID: 28451324; PMCID: PMC5399535.
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Chem Sci. 2017 Mar 01;8(3):2056-2060. doi: 10.1039/c6sc03655d. Epub 2016 Nov 28.

Enabling the controlled assembly of antibody conjugates with a loading of two modules without antibody engineering.

Chemical science

Maximillian T W Lee, Antoine Maruani, Daniel A Richards, James R Baker, Stephen Caddick, Vijay Chudasama

Affiliations

  1. Department of Chemistry , University College London , 20 Gordon Street , London , WC1H 0AJ , UK . Email: [email protected] ; Tel: +44 (0)207 679 2077.

PMID: 28451324 PMCID: PMC5399535 DOI: 10.1039/c6sc03655d

Abstract

The generation of antibody conjugates with a loading of two modules is desirable for a host of reasons. Whilst certain antibody engineering approaches have been useful in the preparation of such constructs, a reliable method based on a native antibody scaffold without the use of enzymes or harsh oxidative conditions has hitherto not been achieved. The use of native antibodies has several advantages in terms of cost, practicality, accessibility, time and overall efficiency. Herein we present a novel, reliable method of furnishing antibody conjugates with a loading of two modules starting from a native antibody scaffold.

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