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Thorat AS, Sonone NA, Choudhari VV, et al. Plant regeneration from cell suspension culture in Saccharum officinarum L. and ascertaining of genetic fidelity through RAPD and ISSR markers. 3 Biotech. 2017;7(1):16doi: 10.1007/s13205-016-0579-3.
Thorat, A. S., Sonone, N. A., Choudhari, V. V., Devarumath, R. M., & Babu, K. H. (2017). Plant regeneration from cell suspension culture in Saccharum officinarum L. and ascertaining of genetic fidelity through RAPD and ISSR markers. 3 Biotech, 7(1), 16. https://doi.org/10.1007/s13205-016-0579-3
Thorat, Avinash S, et al. "Plant regeneration from cell suspension culture in Saccharum officinarum L. and ascertaining of genetic fidelity through RAPD and ISSR markers." 3 Biotech vol. 7,1 (2017): 16. doi: https://doi.org/10.1007/s13205-016-0579-3
Thorat AS, Sonone NA, Choudhari VV, Devarumath RM, Babu KH. Plant regeneration from cell suspension culture in Saccharum officinarum L. and ascertaining of genetic fidelity through RAPD and ISSR markers. 3 Biotech. 2017 May;7(1):16. doi: 10.1007/s13205-016-0579-3. Epub 2017 Apr 08. PMID: 28391480; PMCID: PMC5385170.
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3 Biotech. 2017 May;7(1):16. doi: 10.1007/s13205-016-0579-3. Epub 2017 Apr 08.

Plant regeneration from cell suspension culture in Saccharum officinarum L. and ascertaining of genetic fidelity through RAPD and ISSR markers.

3 Biotech

Avinash S Thorat, Nishant A Sonone, Vrushali V Choudhari, Rachayya M Devarumath, K Harinath Babu

Affiliations

  1. Molecular Biology and Genetic Engineering Section, Vasantdada Sugar Institute, Manjari (Bk), Pune, Maharashtra, India.
  2. Department of Botany, Shivaji University, Kolhapur, Maharashtra, India.
  3. Molecular Biology and Genetic Engineering Section, Vasantdada Sugar Institute, Manjari (Bk), Pune, Maharashtra, India. [email protected].

PMID: 28391480 PMCID: PMC5385170 DOI: 10.1007/s13205-016-0579-3

Abstract

The aim of this study was to produce sugarcane plantlets from cell suspension culture and study its genetic fidelity using molecular markers. The study was carried out using sugarcane varieties Co 86032 and Q117. Callus cultures of both the varieties were optimized using six different callus induction media. After screening the growth response of callus on six different callus induction media, it was observed that medium no. VI supplemented with 500 mg l

Keywords: Callus; Cell suspension culture; Genetic fidelity; ISSR; RAPD; Somaclonal variation; Sugarcane

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