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Jahromi IR, Mehrabani D, Mohammadi A, et al. Emergence of signs of neural cells after exposure of bone marrow-derived mesenchymal stem cells to fetal brain extract. Iran J Basic Med Sci. 2017;20(3):301-307doi: 10.22038/ijbms.2017.8360.
Jahromi, I. R., Mehrabani, D., Mohammadi, A., Seno, M. M., Dianatpour, M., Zare, S., & Tamadon, A. (2017). Emergence of signs of neural cells after exposure of bone marrow-derived mesenchymal stem cells to fetal brain extract. Iranian journal of basic medical sciences, 20(3), 301-307. https://doi.org/10.22038/ijbms.2017.8360
Jahromi, Iman Razeghian, et al. "Emergence of signs of neural cells after exposure of bone marrow-derived mesenchymal stem cells to fetal brain extract." Iranian journal of basic medical sciences vol. 20,3 (2017): 301-307. doi: https://doi.org/10.22038/ijbms.2017.8360
Jahromi IR, Mehrabani D, Mohammadi A, Seno MM, Dianatpour M, Zare S, Tamadon A. Emergence of signs of neural cells after exposure of bone marrow-derived mesenchymal stem cells to fetal brain extract. Iran J Basic Med Sci. 2017 Mar;20(3):301-307. doi: 10.22038/ijbms.2017.8360. PMID: 28392903; PMCID: PMC5378968.
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Iran J Basic Med Sci. 2017 Mar;20(3):301-307. doi: 10.22038/ijbms.2017.8360.

Emergence of signs of neural cells after exposure of bone marrow-derived mesenchymal stem cells to fetal brain extract.

Iranian journal of basic medical sciences

Iman Razeghian Jahromi, Davood Mehrabani, Ali Mohammadi, Mohammad Mahdi Ghahramani Seno, Mehdi Dianatpour, Shahrokh Zare, Amin Tamadon

Affiliations

  1. Cardiovascular Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
  2. Stem Cells Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
  3. Division of Biotechnology, Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
  4. Division of Biotechnology, Department of Pathobiology, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.
  5. Stem Cells Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran; Department of Medical Genetics, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.

PMID: 28392903 PMCID: PMC5378968 DOI: 10.22038/ijbms.2017.8360

Abstract

OBJECTIVES: Nowadays much effort is being invested in order to diagnose the mechanisms involved in neural differentiation. By clarifying this, making desired neural cells

MATERIALS AND METHODS: For this purpose, BM-MSCs were collected from rats and cultured and their mesenchymal properties were confirmed. After exposure of the BM-MSCs to fetal brain extract, the cells were evaluated and harvested at days 3 and 7 after treatment.

RESULTS: The BM-MSCs that were exposed to FBE changed their appearance dramatically from spindle shape to cells with dendrite-like processes. Those neural like processes were absent in the control group. In addition, a neural specific marker, vimentin, was expressed significantly in the treatment group but not in the negative control group.

CONCLUSION: This study presented the FBE as a natural neural differentiation agent, which probably has required factors for making neurons. In addition, vimentin overexpression was observed in the treated group which confirms neuron-like cell differentiation of BM-MSCs after induction.

Keywords: Bone marrow-mesenchymal- stem cells; Differentiation; Fetal brain extract; Neural cell; Vimentin

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