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Vasco MS, Alves LC, Corregidor V, et al. 3D map distribution of metallic nanoparticles in whole cells using MeV ion microscopy. J Microsc. 2017;267(2):227-236doi: 10.1111/jmi.12561.
Vasco, M. S., Alves, L. C., Corregidor, V., Correia, D., Godinho, C. P., Sá-Correia, I., Bettiol, A., Watt, F., & Pinheiro, T. (2017). 3D map distribution of metallic nanoparticles in whole cells using MeV ion microscopy. Journal of microscopy, 267(2), 227-236. https://doi.org/10.1111/jmi.12561
Vasco, M S, et al. "3D map distribution of metallic nanoparticles in whole cells using MeV ion microscopy." Journal of microscopy vol. 267,2 (2017): 227-236. doi: https://doi.org/10.1111/jmi.12561
Vasco MS, Alves LC, Corregidor V, Correia D, Godinho CP, Sá-Correia I, Bettiol A, Watt F, Pinheiro T. 3D map distribution of metallic nanoparticles in whole cells using MeV ion microscopy. J Microsc. 2017 Aug;267(2):227-236. doi: 10.1111/jmi.12561. Epub 2017 Apr 10. PMID: 28394445.
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J Microsc. 2017 Aug;267(2):227-236. doi: 10.1111/jmi.12561. Epub 2017 Apr 10.

3D map distribution of metallic nanoparticles in whole cells using MeV ion microscopy.

Journal of microscopy

M S Vasco, L C Alves, V Corregidor, D Correia, C P Godinho, I Sá-Correia, A Bettiol, F Watt, T Pinheiro

Affiliations

  1. Departamento de Engenharia e Ciências Nucleares, Instituto Superior Técnico, Universidade de Lisboa, Lisboa, Portugal.
  2. Centro de Ciências e Tecnologias Nucleares (C2TN), Instituto Superior Técnico, Universidade de Lisboa, Lisboa, Portugal.
  3. Instituto de Plasmas e Fusão Nuclear (IPFN), Instituto Superior Técnico, Universidade de Lisboa, Lisboa, Portugal.
  4. Instituto de Bioengenharia e Biociencias (IBB), Departamento de Bioengenharia, Instituto Superior Técnico, Universidade de Lisboa, Lisboa, Portugal.
  5. Centre for Ion Beam Applications, Department of Physics, National University of Singapore, Singapore, Singapore.
  6. Instituto de Bioengenharia e Biociências (IBB), Instituto Superior Técnico, Universidade de Lisboa, Lisboa, Portugal.

PMID: 28394445 DOI: 10.1111/jmi.12561

Abstract

In this work, a new tool was developed, the MORIA program that readily translates Rutherford backscattering spectrometry (RBS) output data into visual information, creating a display of the distribution of elements in a true three-dimensional (3D) environment. The program methodology is illustrated with the analysis of yeast Saccharomyces cerevisiae cells, exposed to copper oxide nanoparticles (CuO-NP) and HeLa cells in the presence of gold nanoparticles (Au-NP), using different beam species, energies and nuclear microscopy systems. Results demonstrate that for both cell types, the NP internalization can be clearly perceived. The 3D models of the distribution of CuO-NP in S. cerevisiae cells indicate the nonuniform distribution of NP in the cellular environment and a relevant confinement of CuO-NP to the cell wall. This suggests the impenetrability of certain cellular organelles or compartments for NP. By contrast, using a high-resolution ion beam system, discretized agglomerates of Au-NP were visualized inside the HeLa cell. This is consistent with the mechanism of entry of these NPs in the cellular space by endocytosis enclosed in endosomal vesicles. This approach shows RBS to be a powerful imaging technique assigning to nuclear microscopy unparalleled potential to assess nanoparticle distribution inside the cellular volume.

© 2017 The Authors Journal of Microscopy © 2017 Royal Microscopical Society.

Keywords: 3D imaging; Rutherford backscattering; nanoparticles internalization; nuclear microscopy

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