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Nguyen TTP, Bhandari B, Cichero J, et al. Gastrointestinal digestion of dairy and soy proteins in infant formulas: An in vitro study. Food Res Int. 2015;76:348-358doi: 10.1016/j.foodres.2015.07.030.
Nguyen, T. T. P., Bhandari, B., Cichero, J., & Prakash, S. (2015). Gastrointestinal digestion of dairy and soy proteins in infant formulas: An in vitro study. Food research international (Ottawa, Ont.), 76348-358. https://doi.org/10.1016/j.foodres.2015.07.030
Nguyen, Thao T P, et al. "Gastrointestinal digestion of dairy and soy proteins in infant formulas: An in vitro study." Food research international (Ottawa, Ont.) vol. 76 (2015): 348-358. doi: https://doi.org/10.1016/j.foodres.2015.07.030
Nguyen TTP, Bhandari B, Cichero J, Prakash S. Gastrointestinal digestion of dairy and soy proteins in infant formulas: An in vitro study. Food Res Int. 2015 Oct;76:348-358. doi: 10.1016/j.foodres.2015.07.030. Epub 2015 Jul 31. PMID: 28455014.
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Food Res Int. 2015 Oct;76:348-358. doi: 10.1016/j.foodres.2015.07.030. Epub 2015 Jul 31.

Gastrointestinal digestion of dairy and soy proteins in infant formulas: An in vitro study.

Food research international (Ottawa, Ont.)

Thao T P Nguyen, Bhesh Bhandari, Julie Cichero, Sangeeta Prakash

Affiliations

  1. School of Agriculture and Food Sciences, The University of Queensland, Brisbane, Australia.
  2. School of Pharmacy, Pharmacy Australia Centre of Excellence, The University of Queensland, Brisbane, Australia.
  3. School of Agriculture and Food Sciences, The University of Queensland, Brisbane, Australia. Electronic address: [email protected].

PMID: 28455014 DOI: 10.1016/j.foodres.2015.07.030

Abstract

An in vitro digestion simulating infant gastrointestinal tract studied the digestion of caseins, whey and soy proteins, commonly used in infant formulations, in the presence of proteases only (without lipolytic enzymes). 60min of gastric phase and 120min of intestinal phase coupled with gel electrophoresis, confocal microscopy, mastersizer and pH were employed to monitor the degradation of proteins, microstructure, particle size distribution and pH drop of the digesta through the in vitro digestion process. Obtained results showed around 20% of caseins and almost no components of whey were hydrolysed after 60min in the simulated stomach. In the simulated duodenal phase, 8% of α-lactalbumin was hydrolysed while caseins and β-lactoglobulin completely digested immediately and 30min respectively after addition of duodenal digestive proteases. Overall, soy proteins indicated lower level of hydrolysis than dairy proteins during in vitro infant digestion as observed by SDS-PAGE. The soy protein fractions glycinin and β-conglycinin were partially hydrolysed during the gastrointestinal phase. The observed pH drop confirms that caseins are easily digested in the duodenal phase compared to whey and soy protein. Gastric digestion resulted in a decrease of the particle size of protein aggregates, but no fat coalescence was observed during both gastric and duodenal digestions in the given conditions.

Copyright © 2015 Elsevier Ltd. All rights reserved.

Keywords: Caseins; Confocal microscopy; Particle size; Proteolysis; Soy protein isolate; Whey protein

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