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Sommella E, Pepe G, Pagano F, et al. Detailed polyphenolic profiling of Annurca apple (M. pumila Miller cv Annurca) by a combination of RP-UHPLC and HILIC, both hyphenated to IT-TOF mass spectrometry. Food Res Int. 2015;76:466-477doi: 10.1016/j.foodres.2015.05.044.
Sommella, E., Pepe, G., Pagano, F., Ostacolo, C., Tenore, G. C., Russo, M. T., Novellino, E., Manfra, M., & Campiglia, P. (2015). Detailed polyphenolic profiling of Annurca apple (M. pumila Miller cv Annurca) by a combination of RP-UHPLC and HILIC, both hyphenated to IT-TOF mass spectrometry. Food research international (Ottawa, Ont.), 76466-477. https://doi.org/10.1016/j.foodres.2015.05.044
Sommella, Eduardo, et al. "Detailed polyphenolic profiling of Annurca apple (M. pumila Miller cv Annurca) by a combination of RP-UHPLC and HILIC, both hyphenated to IT-TOF mass spectrometry." Food research international (Ottawa, Ont.) vol. 76 (2015): 466-477. doi: https://doi.org/10.1016/j.foodres.2015.05.044
Sommella E, Pepe G, Pagano F, Ostacolo C, Tenore GC, Russo MT, Novellino E, Manfra M, Campiglia P. Detailed polyphenolic profiling of Annurca apple (M. pumila Miller cv Annurca) by a combination of RP-UHPLC and HILIC, both hyphenated to IT-TOF mass spectrometry. Food Res Int. 2015 Oct;76:466-477. doi: 10.1016/j.foodres.2015.05.044. Epub 2015 May 29. PMID: 28455027.
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Food Res Int. 2015 Oct;76:466-477. doi: 10.1016/j.foodres.2015.05.044. Epub 2015 May 29.

Detailed polyphenolic profiling of Annurca apple (M. pumila Miller cv Annurca) by a combination of RP-UHPLC and HILIC, both hyphenated to IT-TOF mass spectrometry.

Food research international (Ottawa, Ont.)

Eduardo Sommella, Giacomo Pepe, Francesco Pagano, Carmine Ostacolo, Gian Carlo Tenore, Maria Teresa Russo, Ettore Novellino, Michele Manfra, Pietro Campiglia

Affiliations

  1. Department of Pharmacy, School of Pharmacy, University of Salerno, Via Giovanni Paolo II 132, I-84084 Fisciano, SA, Italy.
  2. Department of Pharmacy, University of Naples Federico II, Via D. Montesano 49, I-80131 Napoli, Italy.
  3. Department of Agriculture, Laboratory of Food Chemistry, University of Reggio Calabria Feo di Vito, I-89100 Reggio Calabria, Italy.
  4. Department of Science, University of Basilicata, Viale dell'Ateneo Lucano 10, I-85100 Potenza, Italy.
  5. Department of Pharmacy, School of Pharmacy, University of Salerno, Via Giovanni Paolo II 132, I-84084 Fisciano, SA, Italy. Electronic address: [email protected].

PMID: 28455027 DOI: 10.1016/j.foodres.2015.05.044

Abstract

Annurca apple, a Southern Italian cultivar, possesses not only a particular taste and flavor, different from other types of apple, but also several healthy properties. With the aim to thoroughly elucidate the polyphenolic profile of this variety, listed as Protected Geographical Indication product, an extensive qualitative profiling of Annurca apple polyphenolic peel extract was carried out, by employing a combination of ultra high performance reversed phase (RP-UHPLC) and hydrophilic liquid chromatography (HILIC) coupled to ion trap-time of flight (IT-TOF) mass spectrometry. A total of 63 compounds were tentatively identified, 25 of which not reported in Annurca apple extract so far. Furthermore, thanks to the different selectivity obtained with the HILIC, in combination with accurate mass measurements, an improved separation and detection of procyanidins, was obtained. Moreover, the obtained profiles were compared with those of a conventional variety, such as Red Delicious (RD), highlighting their differences. This work contributes to increase the knowledge about the polyphenolic fingerprint of this typical apple variety.

Copyright © 2015 Elsevier Ltd. All rights reserved.

Keywords: Annurca; HILIC; IT-TOF; Polyphenols; Profiling

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