Front Neurol. 2017 May 03;8:178. doi: 10.3389/fneur.2017.00178. eCollection 2017.

Validation of Repeated Endothelial Function Measurements Using EndoPAT in Stroke.

Frontiers in neurology

Aina S Hansen, Jawad H Butt, Sonja Holm-Yildiz, William Karlsson, Christina Kruuse

PMID: 28515707 PMCID: PMC5413501 DOI: 10.3389/fneur.2017.00178

Abstract

BACKGROUND: Decreased endothelial function (EF) may be a prognostic marker for stroke. Measuring pharmacological effects on EF may be of interest in the development of personalized medicine for stroke prevention. In this study, we assessed the reliability of repeated EF measurements using a pulse amplitude tonometry technology in acute stroke patients. Similarly, reliability was tested in healthy subjects devoid of vascular disease to estimate reactivity and reliability in a younger non-stroke population.

MATERIALS AND METHODS: EF was assessed using the EndoPAT2000 in 20 healthy volunteers (men 50%, mean age 35.85 ± 3.47 years) and 21 stroke patients (men 52%, mean age 66.38 ± 2.85 years, and mean NIHSS 4.09 ± 0.53) under standardized conditions. EF was measured as the reactive hyperemia index (RHI), logarithm of RHI (lnRHI), and Framingham RHI (fRHI). Measurements were separated by 1.5 and 24 h to assess same-day and day-to-day reliability, respectively.

RESULTS: Fair to moderate correlations of measurements [intraclass correlation coefficient (ICC)

CONCLUSION: Repeated measurements of fRHI in stroke patients show moderate reliability on same-day and substantial on day-to-day measurements. Likewise, in healthy subjects there was substantial reliability on day-to-day measurement, but only moderate on same-day measurements. In general, day-to-day correlation of repeated EF measurements was far better than that of same-day measurements, which ranged from poor to moderate depending on the specific outcome measure of EF. A possible carryover effect should be considered if same-day repeated testing of drug effects is applied in future studies.

Keywords: EndoPAT; endothelial function; stroke; validation; vasomotor reactivity

References

1. J Vasc Interv Neurol. 2012 Jun;5(1):33-9 - PubMed
2. J Am Coll Cardiol. 1999 Jul;34(1):146-54 - PubMed
3. Stroke. 2003 Sep;34(9):2132-7 - PubMed
4. Stroke. 1993 Jan;24(1):35-41 - PubMed
5. Circulation. 2001 Nov 27;104(22):2673-8 - PubMed
6. Circulation. 2000 Mar 7;101(9):948-54 - PubMed
7. Trends Cardiovasc Med. 2009 Jan;19(1):6-11 - PubMed
8. Hypertension. 2011 Mar;57(3):390-6 - PubMed
9. J Am Coll Cardiol. 2013 Sep 17;62(12 ):1114-21 - PubMed
10. Int J Vasc Med. 2012;2012:904141 - PubMed
11. Curr Pharm Des. 2008;14 (35):3715-22 - PubMed
12. Acta Physiol Scand. 1976 Aug;97(4):447-56 - PubMed
13. Circulation. 2007 Mar 13;115(10):1285-95 - PubMed
14. Curr Opin Endocrinol Diabetes Obes. 2007 Oct;14(5):365-9 - PubMed
15. Int J Cardiol. 2013 Sep 30;168(2):1280-5 - PubMed
16. Vasc Med. 2012 Feb;17(1):29-36 - PubMed
17. Vasc Med. 2012 Apr;17 (2):79-84 - PubMed
18. Am J Cardiol. 2007 May 1;99(9):1258-62 - PubMed
19. Circulation. 2008 May 13;117(19):2467-74 - PubMed
20. J Am Coll Cardiol. 2004 Dec 7;44(11):2137-41 - PubMed
21. Circulation. 2000 Apr 25;101(16):1899-906 - PubMed
22. Cerebrovasc Dis. 2012;33(1):37-46 - PubMed
23. Int J Cardiol. 2009 May 1;134(1):52-8 - PubMed
24. Circ Cardiovasc Imaging. 2011 Jul;4(4):371-80 - PubMed
25. J Appl Physiol (1985). 2008 Aug;105(2):427-32 - PubMed
26. Hypertension. 2011 Mar;57(3):363-9 - PubMed
27. J Behav Med. 2008 Apr;31(2):137-43 - PubMed
28. J Neurol Neurosurg Psychiatry. 2007 Jul;78(7):702-6 - PubMed
29. Biometrics. 1977 Mar;33(1):159-74 - PubMed
30. J Am Coll Cardiol. 2002 Jan 16;39(2):257-65 - PubMed
31. J Appl Physiol (1985). 2006 Aug;101(2):545-8 - PubMed
32. Cerebrovasc Dis. 2011;32(2):155-62 - PubMed
33. J Nutr. 2008 Oct;138(10):1910-4 - PubMed
34. Clin Cardiol. 2009 Dec;32(12):700-4 - PubMed
35. Circulation. 2012 Aug 7;126(6):753-67 - PubMed
36. Am J Cardiol. 2008 Jan 1;101(1):82-8 - PubMed

Publication Types

• Journal Article