Display options
Cite
Gong J, Wang J, Tian Y, et al. Expression of tubulin folding cofactor B in mouse hepatic ischemia-reperfusion injury. Biomed Rep. 2017;6(5):525-531doi: 10.3892/br.2017.891.
Gong, J., Wang, J., Tian, Y., Zhang, J., Liang, W., Li, Z., Yu, J., Tang, B., & He, S. (2017). Expression of tubulin folding cofactor B in mouse hepatic ischemia-reperfusion injury. Biomedical reports, 6(5), 525-531. https://doi.org/10.3892/br.2017.891
Gong, Jianhua, et al. "Expression of tubulin folding cofactor B in mouse hepatic ischemia-reperfusion injury." Biomedical reports vol. 6,5 (2017): 525-531. doi: https://doi.org/10.3892/br.2017.891
Gong J, Wang J, Tian Y, Zhang J, Liang W, Li Z, Yu J, Tang B, He S. Expression of tubulin folding cofactor B in mouse hepatic ischemia-reperfusion injury. Biomed Rep. 2017 May;6(5):525-531. doi: 10.3892/br.2017.891. Epub 2017 Apr 11. PMID: 28515911; PMCID: PMC5431315.
Copy Download .nbib Format: NLM
Share it on

Biomed Rep. 2017 May;6(5):525-531. doi: 10.3892/br.2017.891. Epub 2017 Apr 11.

Expression of tubulin folding cofactor B in mouse hepatic ischemia-reperfusion injury.

Biomedical reports

Jianhua Gong, Junyi Wang, Yu Tian, Jing Zhang, Wenjin Liang, Zeming Li, Jidong Yu, Bo Tang, Songqing He

Affiliations

  1. Department of Hepatobiliary Surgery and Laboratory, Affiliated Hospital of Guilin Medical University, Guilin, Guangxi 541001, P.R. China.
  2. Division of Hepatobiliary and Pancreatic Surgery, Department of Surgery, The Second Hospital of Dalian Medical University, Dalian, Liaoning 116027, P.R. China.

PMID: 28515911 PMCID: PMC5431315 DOI: 10.3892/br.2017.891

Abstract

The aim of the present study was to investigate the association between tubulin folding cofactor B (TBCB) expression and ischemia-reperfusion injury (IRI) in mice. A total of 48 C57BL/6 mice were randomly divided into a control group (Sham, n=6) and an ischemia-reperfusion group (n=42). The ischemia-reperfusion group was further divided into 6 subgroups as per different times after reperfusion (2, 4, 6, 8, 12 and 24 h), with 7 mice per subgroup. A hepatic IRI model was established in mice by clamping the hepatic hilum. Morphology, serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), interleukin 6 (IL-6) and tumor necrosis factor-α (TNF-α), and the expression level of TBCB were detected. Compared with the control group, the livers from the ischemia-reperfusion group were significantly changed, particularly at 12 h following ischemia-reperfusion, with obvious hepatic cell degeneration and necrosis. The ALT, AST, IL-6 and TNF-α levels in the sera of the mice in the hepatic ischemia-reperfusion group were increased at all time points following ischemia-reperfusion, and were the highest at 12 h, demonstrating statistically significant differences when compared with the control group (P<0.05). Furthermore, the expression levels of TBCB, TNF-α and IL-6 were significantly increased at all time-points following ischemia-reperfusion, and were the most significant at 12 h. At 24 h following ischemia-reperfusion, the expression levels had decreased. The present study indicated that TBCB expression is associated with TNF-α and IL-6 expression levels in mice with hepatic ischemia-reperfusion, and may be key in the development of liver injury during ischemia-reperfusion in mice.

Keywords: cell microtubule; cytoskeleton; ischemia-reperfusion injury; tubulin folding cofactor B

References

  1. Circ Res. 2000 Jan 7-21;86(1):30-6 - PubMed
  2. Naunyn Schmiedebergs Arch Pharmacol. 2012 May;385(5):465-71 - PubMed
  3. Int Braz J Urol. 2014 Jul-Aug;40(4):553-61 - PubMed
  4. Naunyn Schmiedebergs Arch Pharmacol. 2001 Sep;364(3):276-80 - PubMed
  5. Trends Cell Biol. 1997 Dec;7(12):479-84 - PubMed
  6. Gastroenterology. 2000 Jan;118(1):183-91 - PubMed
  7. Med Sci Monit. 2002 Nov;8(11):BR444-53 - PubMed
  8. Methods. 2001 Dec;25(4):402-8 - PubMed
  9. Neuron. 2003 Apr 24;38(2):177-85 - PubMed
  10. FASEB J. 2001 May;15(7):1230-2 - PubMed
  11. Biochemistry. 1999 Apr 27;38(17):5490-8 - PubMed
  12. World J Gastrointest Surg. 2014 Jul 27;6(7):122-8 - PubMed
  13. Cell. 1995 Aug 11;82(3):425-34 - PubMed
  14. Science. 1997 Oct 10;278(5336):294-8 - PubMed
  15. J Immunol. 1986 Jun 1;136(11):4220-5 - PubMed
  16. Pharmacol Rep. 2014 Aug;66(4):647-52 - PubMed
  17. J Surg Res. 2012 Aug;176(2):614-20 - PubMed
  18. Cells. 2014 Sep 11;3(3):899-913 - PubMed
  19. Exp Cell Res. 2007 Feb 1;313(3):425-36 - PubMed
  20. J Neurochem. 2007 Mar;100(6):1680-7 - PubMed
  21. Am J Pathol. 1987 Nov;129(2):327-44 - PubMed
  22. Transplantation. 1997 Nov 27;64(10):1398-403 - PubMed
  23. Am J Physiol Lung Cell Mol Physiol. 2005 Apr;288(4):L749-60 - PubMed
  24. Annu Rev Cell Dev Biol. 2008;24:1-28 - PubMed
  25. Ann Surg. 1908 Oct;48(4):541-9 - PubMed
  26. Transplantation. 2014 Jun 15;97(11):1102-9 - PubMed

Publication Types