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Elkady A, Iijima S, Aboulfotuh S, et al. Characteristics of escape mutations from occult hepatitis B virus infected patients with hematological malignancies in South Egypt. World J Hepatol. 2017;9(9):477-486doi: 10.4254/wjh.v9.i9.477.
Elkady, A., Iijima, S., Aboulfotuh, S., Mostafa Ali, E., Sayed, D., Abdel-Aziz, N. M., Ali, A. M., Murakami, S., Isogawa, M., & Tanaka, Y. (2017). Characteristics of escape mutations from occult hepatitis B virus infected patients with hematological malignancies in South Egypt. World journal of hepatology, 9(9), 477-486. https://doi.org/10.4254/wjh.v9.i9.477
Elkady, Abeer, et al. "Characteristics of escape mutations from occult hepatitis B virus infected patients with hematological malignancies in South Egypt." World journal of hepatology vol. 9,9 (2017): 477-486. doi: https://doi.org/10.4254/wjh.v9.i9.477
Elkady A, Iijima S, Aboulfotuh S, Mostafa Ali E, Sayed D, Abdel-Aziz NM, Ali AM, Murakami S, Isogawa M, Tanaka Y. Characteristics of escape mutations from occult hepatitis B virus infected patients with hematological malignancies in South Egypt. World J Hepatol. 2017 Mar 28;9(9):477-486. doi: 10.4254/wjh.v9.i9.477. PMID: 28396718; PMCID: PMC5368625.
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World J Hepatol. 2017 Mar 28;9(9):477-486. doi: 10.4254/wjh.v9.i9.477.

Characteristics of escape mutations from occult hepatitis B virus infected patients with hematological malignancies in South Egypt.

World journal of hepatology

Abeer Elkady, Sayuki Iijima, Sahar Aboulfotuh, Elsayed Mostafa Ali, Douaa Sayed, Nashwa M Abdel-Aziz, Amany M Ali, Shuko Murakami, Masanori Isogawa, Yasuhito Tanaka

Affiliations

  1. Abeer Elkady, Department of Clinical and Chemical Pathology, Medical School of South Valley University, Qena 83523, Egypt.

PMID: 28396718 PMCID: PMC5368625 DOI: 10.4254/wjh.v9.i9.477

Abstract

AIM: To investigate the prevalence and virological characteristics of occult hepatitis B virus (HBV) infections in patients with hematological malignancies in South Egypt.

METHODS: Serum samples were collected from 165 patients with hematological malignancies to monitor titers of HBV DNA, hepatitis B surface antigen (HBsAg), and antibodies to HBV core (anti-HBc) and surface antigens. Serum samples negative for HBsAg and positive for anti-HBc were subjected to nucleic acid extraction and HBV DNA detection by real-time polymerase chain reaction. DNA sequences spanning the S region were analyzed in cases with occult HBV infection.

RESULTS: HBV DNA was detected in 23 (42.6%) of 54 patients with hematological malignancies who were HBsAg negative, but anti-HBc positive, suggesting the presence of occult HBV infection. The complete HBV genome was retrieved from 6 occult HBV patients, and P120T and S143L were detected in 3 and 2 cases, respectively. Site directed mutagenesis was done to produce 1.24-fold genotype D clones with amino acid mutations T120 and L143. The

CONCLUSION: Occult HBV infection is common in patients with hematological malignancies and associated with P120T and S143L mutations. 120T mutation impairs the detection of HBsAg by CLEIA.

Keywords: Escape mutation; Hematological malignancies; Occult hepatitis B infection

Conflict of interest statement

Conflict-of-interest statement: Authors do not have any financial or personal relationship with other people or organizations that could inappropriately influence their work.

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