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Alam J, Choi YS, Koh JH, et al. Detection of Autoantibodies against Aquaporin-1 in the Sera of Patients with Primary Sjögren's Syndrome. Immune Netw. 2017;17(2):103-109doi: 10.4110/in.2017.17.2.103.
Alam, J., Choi, Y. S., Koh, J. H., Kwok, S. K., Park, S. H., Song, Y. W., Park, K., & Choi, Y. (2017). Detection of Autoantibodies against Aquaporin-1 in the Sera of Patients with Primary Sjögren's Syndrome. Immune network, 17(2), 103-109. https://doi.org/10.4110/in.2017.17.2.103
Alam, Jehan, et al. "Detection of Autoantibodies against Aquaporin-1 in the Sera of Patients with Primary Sjögren's Syndrome." Immune network vol. 17,2 (2017): 103-109. doi: https://doi.org/10.4110/in.2017.17.2.103
Alam J, Choi YS, Koh JH, Kwok SK, Park SH, Song YW, Park K, Choi Y. Detection of Autoantibodies against Aquaporin-1 in the Sera of Patients with Primary Sjögren's Syndrome. Immune Netw. 2017 Apr;17(2):103-109. doi: 10.4110/in.2017.17.2.103. Epub 2017 Apr 20. PMID: 28458621; PMCID: PMC5407981.
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Immune Netw. 2017 Apr;17(2):103-109. doi: 10.4110/in.2017.17.2.103. Epub 2017 Apr 20.

Detection of Autoantibodies against Aquaporin-1 in the Sera of Patients with Primary Sjögren's Syndrome.

Immune network

Jehan Alam, Yun Sik Choi, Jung Hee Koh, Seung-Ki Kwok, Sung-Hwan Park, Yeong Wook Song, Kyungpyo Park, Youngnim Choi

Affiliations

  1. School of Dentistry and Dental Research Institute, Seoul National University, Seoul 03080, Korea.
  2. Seoul St. Mary's Hospital, The Catholic University of Korea, Seoul 06591, Korea.
  3. College of Medicine, Seoul National University, Seoul 03080, Korea.

PMID: 28458621 PMCID: PMC5407981 DOI: 10.4110/in.2017.17.2.103

Abstract

The pathophysiology of glandular dysfunction in Sjögren's syndrome (SS) has not been fully elucidated. Previously, we reported the presence of autoantibodies to AQP-5 in patients with SS, which was associated with a low resting salivary flow. The purpose of this study was to investigate the presence of anti-AQP1 autoantibodies. To detect anti-AQP1 autoantibodies, cell-based indirect immunofluorescence assay was developed using MDCK cells that overexpressed human AQP1. By screening 112 SS and 52 control sera, anti-AQP1 autoantibodies were detected in 27.7% of the SS but in none of the control sera. Interestingly, the sera that were positive for anti-AQP1 autoantibodies also contained anti-AQP5 autoantibodies in the previous study. Different from anti-AQP5 autoantibodies, the presence of anti-AQP1 autoantibodies was not associated with the salivary flow rate. Although anti-AQP1 autoantibodies are not useful as a diagnostic marker, the presence of autoantibodies to AQP1 may be an obstacle to AQP1 gene therapy for SS.

Keywords: Aquaporin 1; Autoantibodies; Fluorescent antibody technique; Sjögren's syndrome

Conflict of interest statement

CONFLICTS OF INTEREST: The authors declare that there are no conflicts of interest.

References

  1. Lancet. 2005 Jul 23-29;366(9482):321-31 - PubMed
  2. Curr Pharm Biotechnol. 2012 Aug;13(10):2063-70 - PubMed
  3. J Dent Res. 1982 Oct;61(10):1158-62 - PubMed
  4. Lab Invest. 2002 Nov;82(11):1547-52 - PubMed
  5. Clin Rheumatol. 2006 Nov;25(6):891-4 - PubMed
  6. Ann Rheum Dis. 2002 Jun;61(6):554-8 - PubMed
  7. Oral Dis. 2015 Jan;21(1):e114-20 - PubMed
  8. Arthritis Care Res (Hoboken). 2012 Apr;64(4):475-87 - PubMed
  9. Proc Natl Acad Sci U S A. 1997 Apr 1;94(7):3268-73 - PubMed
  10. Int J Mol Sci. 2016 Jan 27;17 (2):null - PubMed
  11. PLoS One. 2013;8(1):e53113 - PubMed
  12. J Med Invest. 2009;56 Suppl:192-6 - PubMed
  13. Clin Exp Rheumatol. 2015 Mar-Apr;33(2):259-71 - PubMed
  14. J Neuroimmunol. 2014 Aug 15;273(1-2):103-10 - PubMed
  15. Proc Natl Acad Sci U S A. 2016 May 17;113(20):5694-9 - PubMed
  16. Immunol Res. 2016 Aug;64(4):848-56 - PubMed
  17. Periodontol 2000. 2016 Feb;70(1):11-25 - PubMed
  18. Monogr Oral Sci. 2014;24:14-29 - PubMed
  19. Am J Physiol Cell Physiol. 2004 Mar;286(3):C529-37 - PubMed
  20. PLoS One. 2013 Sep 23;8(9):e74773 - PubMed
  21. Proc Natl Acad Sci U S A. 2012 Nov 20;109 (47):19403-7 - PubMed
  22. J Neurol Sci. 2010 Apr 15;291(1-2):52-6 - PubMed
  23. J Biol Chem. 1999 Jul 16;274(29):20071-4 - PubMed
  24. Proc Natl Acad Sci U S A. 1998 Jun 23;95(13):7538-43 - PubMed

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