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Caron J, Cudennec B, Domenger D, et al. Simulated GI digestion of dietary protein: Release of new bioactive peptides involved in gut hormone secretion. Food Res Int. 2016;89:382-390doi: 10.1016/j.foodres.2016.08.033.
Caron, J., Cudennec, B., Domenger, D., Belguesmia, Y., Flahaut, C., Kouach, M., Lesage, J., Goossens, J. F., Dhulster, P., & Ravallec, R. (2016). Simulated GI digestion of dietary protein: Release of new bioactive peptides involved in gut hormone secretion. Food research international (Ottawa, Ont.), 89382-390. https://doi.org/10.1016/j.foodres.2016.08.033
Caron, Juliette, et al. "Simulated GI digestion of dietary protein: Release of new bioactive peptides involved in gut hormone secretion." Food research international (Ottawa, Ont.) vol. 89 (2016): 382-390. doi: https://doi.org/10.1016/j.foodres.2016.08.033
Caron J, Cudennec B, Domenger D, Belguesmia Y, Flahaut C, Kouach M, Lesage J, Goossens JF, Dhulster P, Ravallec R. Simulated GI digestion of dietary protein: Release of new bioactive peptides involved in gut hormone secretion. Food Res Int. 2016 Nov;89:382-390. doi: 10.1016/j.foodres.2016.08.033. Epub 2016 Aug 27. PMID: 28460928.
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Food Res Int. 2016 Nov;89:382-390. doi: 10.1016/j.foodres.2016.08.033. Epub 2016 Aug 27.

Simulated GI digestion of dietary protein: Release of new bioactive peptides involved in gut hormone secretion.

Food research international (Ottawa, Ont.)

Juliette Caron, Benoit Cudennec, Dorothée Domenger, Yanath Belguesmia, Christophe Flahaut, Mostafa Kouach, Jean Lesage, Jean-François Goossens, Pascal Dhulster, Rozenn Ravallec

Affiliations

  1. Univ. Lille, EA 7394- ICV - Institut Charles Viollette, F-59000 Lille, France.
  2. Univ. Lille, EA 7394- ICV - Institut Charles Viollette, F-59000 Lille, France. Electronic address: [email protected].
  3. CUMA, Faculté de Pharmacie, Lille, France.
  4. Univ. Lille, EA 4489, Unité Environnement Périnatal et Croissance - Équipe Dénutritions Maternelles Périnatales, F-59000 Lille, France.

PMID: 28460928 DOI: 10.1016/j.foodres.2016.08.033

Abstract

Dietary proteins have been reported to induce a strong feeling of satiety that has been partially explained by gut hormone level increase. Up to date, various protein hydrolysates have demonstrated in vitro and in vivo their potential to stimulate gut hormone secretion related to food intake decrease and their mechanisms of action have just started to be resolved. In this context, this study aimed at identifying new peptide sequences involved in gut hormone secretion released by protein in vitro gastrointestinal digestion. Targeted gut hormones were Cholecystokinin (CCK) and Glucagon-Like Peptide 1 (GLP-1). The activity of DPP-IV was also considered as it strongly modulates GLP-1 action. In a previous study, simulated digestion of dietary protein has generated hydrolysates with enhancing effect on CCK and GLP-1 secretion in STC-1 cells as well as DPP-IV inhibitory properties. Successive purification steps were performed to isolate peptide fractions involved in these bioactivities whose sequence was determined by LC-MS-MS. Three peptide sequences ANVST, TKAVEH and KAAVT were pointed out for their stimulating effects on GLP-1 secretion. The sequence VAAA was isolated for its DPP-IV inhibitory properties. Two peptide groups were strongly involved in CCK release sharing a certain occurrence of aromatic amino acid residues.

Copyright © 2016 Elsevier Ltd. All rights reserved.

Keywords: Bioactive peptides; DPP-IV; Enteroendocrine cells; Gut hormones; Protein digestion

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