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Condorelli RA, La Vignera S, Giacone F, et al. Nicotine Effects and Receptor Expression on Human Spermatozoa: Possible Neuroendocrine Mechanism. Front Physiol. 2017;8:177doi: 10.3389/fphys.2017.00177.
Condorelli, R. A., La Vignera, S., Giacone, F., Iacoviello, L., Mongioì, L. M., Li Volti, G., Barbagallo, I., Avola, R., & Calogero, A. E. (2017). Nicotine Effects and Receptor Expression on Human Spermatozoa: Possible Neuroendocrine Mechanism. Frontiers in physiology, 8177. https://doi.org/10.3389/fphys.2017.00177
Condorelli, Rosita A, et al. "Nicotine Effects and Receptor Expression on Human Spermatozoa: Possible Neuroendocrine Mechanism." Frontiers in physiology vol. 8 (2017): 177. doi: https://doi.org/10.3389/fphys.2017.00177
Condorelli RA, La Vignera S, Giacone F, Iacoviello L, Mongioì LM, Li Volti G, Barbagallo I, Avola R, Calogero AE. Nicotine Effects and Receptor Expression on Human Spermatozoa: Possible Neuroendocrine Mechanism. Front Physiol. 2017 Mar 28;8:177. doi: 10.3389/fphys.2017.00177. eCollection 2017. PMID: 28400736; PMCID: PMC5368220.
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Front Physiol. 2017 Mar 28;8:177. doi: 10.3389/fphys.2017.00177. eCollection 2017.

Nicotine Effects and Receptor Expression on Human Spermatozoa: Possible Neuroendocrine Mechanism.

Frontiers in physiology

Rosita A Condorelli, Sandro La Vignera, Filippo Giacone, Linda Iacoviello, Laura M Mongioì, Giovanni Li Volti, Ignazio Barbagallo, Roberto Avola, Aldo E Calogero

Affiliations

  1. Department of Clinical and Experimental Medicine, University of Catania Catania, Italy.
  2. Department of Biomedical Sciences and Biotechnology, University of Catania Catania, Italy.
  3. Department of Drug Sciences, University of Catania Catania, Italy.

PMID: 28400736 PMCID: PMC5368220 DOI: 10.3389/fphys.2017.00177

Abstract

The aim of this experimental study was to investigate the mechanism by which nicotine (NIC) alters spermatozoa and to evaluate the expression of nicotinic receptors (nAChR) subunits in human spermatozoa. We analyzed 30 healthy normozoospermic men. Spermatozoa were incubated with NIC 100 ng/ml and the nAChR antagonist, hexamethonium (HEX) (0, 100, 1,000, 10,000 ng/ml) for 3 and 24 h. The following sperm parameters evaluated: (a) progressive motility; (b) mitochondrial membrane potential (MMP); (c) chromatin compactness; (d) externalization of phosphatidylserine (PS); (e) late apoptosis; (f) viability; (g) DNA fragmentation; (h) degree of lipid peroxidation (LP) by flow cytometry; (i) nAChR subunits expression by quantitative Real Time PCR and (j) protein expression evaluation by Western blot analysis. HEX fully antagonized the effects of NIC both after 3 and 24 h of incubation with significant improvement (

Keywords: acetylcholine; hexamethonium; neuroendocrine mechanism; nicotinic receptor; spermatozoa

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