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Gallo A, Landi R, Rubino V, et al. Oxytetracycline induces DNA damage and epigenetic changes: a possible risk for human and animal health?. PeerJ. 2017;5:e3236doi: 10.7717/peerj.3236.
Gallo, A., Landi, R., Rubino, V., Di Cerbo, A., Giovazzino, A., Palatucci, A. T., Centenaro, S., Guidetti, G., Canello, S., Cortese, L., Ruggiero, G., Alessandrini, A., & Terrazzano, G. (2017). Oxytetracycline induces DNA damage and epigenetic changes: a possible risk for human and animal health?. PeerJ, 5e3236. https://doi.org/10.7717/peerj.3236
Gallo, Adriana, et al. "Oxytetracycline induces DNA damage and epigenetic changes: a possible risk for human and animal health?." PeerJ vol. 5 (2017): e3236. doi: https://doi.org/10.7717/peerj.3236
Gallo A, Landi R, Rubino V, Di Cerbo A, Giovazzino A, Palatucci AT, Centenaro S, Guidetti G, Canello S, Cortese L, Ruggiero G, Alessandrini A, Terrazzano G. Oxytetracycline induces DNA damage and epigenetic changes: a possible risk for human and animal health?. PeerJ. 2017 Apr 27;5:e3236. doi: 10.7717/peerj.3236. eCollection 2017. PMID: 28462039; PMCID: PMC5410137.
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PeerJ. 2017 Apr 27;5:e3236. doi: 10.7717/peerj.3236. eCollection 2017.

Oxytetracycline induces DNA damage and epigenetic changes: a possible risk for human and animal health?.

PeerJ

Adriana Gallo, Rosaria Landi, Valentina Rubino, Alessandro Di Cerbo, Angela Giovazzino, Anna Teresa Palatucci, Sara Centenaro, Gianandrea Guidetti, Sergio Canello, Laura Cortese, Giuseppina Ruggiero, Andrea Alessandrini, Giuseppe Terrazzano

Affiliations

  1. Institute of Experimental Endocrinology and Oncology (IEOS), National Research Council (CNR), Naples, Italy.
  2. Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, Naples, Italy.
  3. Department of Translational Medical Sciences, University of Naples Federico II, Naples, Italy.
  4. Department of Physics, Informatics and Mathematics, University of Modena and Reggio Emilia, Modena, Italy.
  5. PhD School of Science, University of Basilicata, Potenza, Italy.
  6. Division of Research and Development, Sanypet SpA, Padova, Italy.
  7. Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, Naples, Italy.
  8. National Research Council (CNR), Nanoscience Istitute, Modena, Italy.
  9. Department of Science, University of Basilicata, Potenza, Italy.

PMID: 28462039 PMCID: PMC5410137 DOI: 10.7717/peerj.3236

Abstract

BACKGROUND: Oxytetracycline (OTC), which is largely employed in zootechnical and veterinary practices to ensure wellness of farmed animals, is partially absorbed within the gastrointestinal tract depositing in several tissues. Therefore, the potential OTC toxicity is relevant when considering the putative risk derived by the entry and accumulation of such drug in human and pet food chain supply. Despite scientific literature highlights several OTC-dependent toxic effects on human and animal health, the molecular mechanisms of such toxicity are still poorly understood.

METHODS: Here, we evaluated DNA damages and epigenetic alterations by quantitative reverse transcription polymerase chain reaction, quantitative polymerase chain reaction, chromatin immuno-precipitation and Western blot analysis.

RESULTS: We observed that human peripheral blood mononuclear cells (PBMCs) expressed DNA damage features (activation of ATM and p53, phosphorylation of H2AX and modifications of histone H3 methylation of lysine K4 in the chromatin) after the

DISCUSSION: Our data reveal an unexpected biological

Keywords: DNA damage; Drug toxicity; Epigenetics; Immune pharmacology; Inflammatory response

Conflict of interest statement

None of the authors have financial or personal relationships with other people or organisations that could inappropriately influence or bias the content of the paper. This research was performed in co

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