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Front Endocrinol (Lausanne). 2017 May 31;8:121. doi: 10.3389/fendo.2017.00121. eCollection 2017.

Quantifying Biochemical Alterations in Brown and Subcutaneous White Adipose Tissues of Mice Using Fourier Transform Infrared Widefield Imaging.

Frontiers in endocrinology

Ebrahim Aboualizadeh, Owen T Carmichael, Ping He, Diana C Albarado, Christopher D Morrison, Carol J Hirschmugl

Affiliations

  1. Department of Physics, University of Wisconsin-Milwaukee, Milwaukee, WI, United States.
  2. Pennington Biomedical Research Center, Baton Rouge, LA, United States.

PMID: 28620356 PMCID: PMC5450226 DOI: 10.3389/fendo.2017.00121

Abstract

Stimulating increased thermogenic activity in adipose tissue is an important biological target for obesity treatment, and label-free imaging techniques with the potential to quantify stimulation-associated biochemical changes to the adipose tissue are highly sought after. In this study, we used spatially resolved Fourier transform infrared (FTIR) imaging to quantify biochemical changes caused by cold exposure in the brown and subcutaneous white adipose tissues (BAT and s-WAT) of 6 week-old C57BL6 mice exposed to 30°C (

Keywords: Fourier transform infrared imaging; adipose thermogenic markers; brown adipose tissue; obesity; spectroscopy; subcutaneous white adipose tissue

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