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Lawley B, Munro K, Hughes A, et al. Differentiation of . PeerJ. 2017;5:e3375doi: 10.7717/peerj.3375.
Lawley, B., Munro, K., Hughes, A., Hodgkinson, A. J., Prosser, C. G., Lowry, D., Zhou, S. J., Makrides, M., Gibson, R. A., Lay, C., Chew, C., Lee, P. S., Wong, K. H., & Tannock, G. W. (2017). Differentiation of . PeerJ, 5e3375. https://doi.org/10.7717/peerj.3375
Lawley, Blair, et al. "Differentiation of ." PeerJ vol. 5 (2017): e3375. doi: https://doi.org/10.7717/peerj.3375
Lawley B, Munro K, Hughes A, Hodgkinson AJ, Prosser CG, Lowry D, Zhou SJ, Makrides M, Gibson RA, Lay C, Chew C, Lee PS, Wong KH, Tannock GW. Differentiation of . PeerJ. 2017 May 25;5:e3375. doi: 10.7717/peerj.3375. eCollection 2017. PMID: 28560114; PMCID: PMC5446769.
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PeerJ. 2017 May 25;5:e3375. doi: 10.7717/peerj.3375. eCollection 2017.

Differentiation of .

PeerJ

Blair Lawley, Karen Munro, Alan Hughes, Alison J Hodgkinson, Colin G Prosser, Dianne Lowry, Shao J Zhou, Maria Makrides, Robert A Gibson, Christophe Lay, Charmaine Chew, Pheng Soon Lee, Khai Hong Wong, Gerald W Tannock

Affiliations

  1. Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand.
  2. AgResearch, Ruakura, Hamilton, New Zealand.
  3. Dairy Goat Cooperative (NZ) Ltd., Hamilton, New Zealand.
  4. Women's and Children's Health Research Institute, Adelaide, Australia.
  5. School of Agriculture, Food and Wine, University of Adelaide, Adelaide, Australia.
  6. Healthy Mothers, Babies and Children, South Australian Health and Medical Research Institute, Adelaide, Australia.
  7. Danone Nutricia Research, Biopolis, Singapore.
  8. Mead Johnson Nutrition, Marina Bay, Singapore.
  9. Riddet Institute Centre of Research Excellence, Palmerston North, New Zealand.
  10. Microbiome Otago, University of Otago, Dunedin, New Zealand.

PMID: 28560114 PMCID: PMC5446769 DOI: 10.7717/peerj.3375

Abstract

BACKGROUND: Members of the genus

METHODS: We developed a qPCR method targeting the sialidase gene (subsp.

RESULTS: A pangenomic search of the NCBI genomic database showed that the PCR primers/probes targeted only the respective genes of the two subspecies. The primers/probes showed total specificity when tested against DNA extracted from the gold standard strains (type cultures) of bifidobacterial species detected in infant feces. Use of the qPCR method with DNA extracted from the feces of infants of different ages, delivery method and nutrition, showed that subsp.

DISCUSSION: Our qPCR method differentiates

Keywords: Bifidobacterium; Functional gene; Infantis; Longum; qPCR

Conflict of interest statement

The authors declare there are no competing interests. Colin G. Prosser and Dianne Lowry are employees of Dairy Goat Cooperative (NZ) Ltd., Hamilton, New Zealand. Pheng Soon Lee and Khai Hong Wong are

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