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Front Immunol. 2017 May 05;8:494. doi: 10.3389/fimmu.2017.00494. eCollection 2017.

Advances in the Isolation of Specific Monoclonal Rabbit Antibodies.

Frontiers in immunology

Zaibao Zhang, Huijuan Liu, Qian Guan, Lei Wang, Hongyu Yuan

Affiliations

  1. Institute for Conservation and Utilization of Agro-Bioresources in Dabie Mountains, Xinyang Normal University, Xinyang, China.
  2. College of Life Science, Xinyang Normal University, Xinyang, China.
  3. State Key Laboratory of Organ Failure Research, National Clinical Research Center for Kidney Disease, Division of Nephrology, Nanfang Hospital, Southern Medical University, Guangzhou, China.

PMID: 28529510 PMCID: PMC5418221 DOI: 10.3389/fimmu.2017.00494

Abstract

The rabbit monoclonal antibodies (mAbs) have advantages in pharmaceuticals and diagnostics with high affinity and specificity. During the past decade, many techniques have been developed for isolating rabbit mAbs, including single B cell antibody technologies. This review describes the basic characterization of rabbit antibody repertoire and summarizes methods of hybridoma technologies, phage display platform, and single B cell antibody technologies. With advances in antibody function and repertoire analysis, rabbit mAbs will be widely used in therapeutic applications in the coming years.

Keywords: hybridoma; phage display; rabbit antibody repertoire; rabbit monoclonal antibody; single B cell antibody technology

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