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Goswami PP, Chand G, Prasad NS, et al. Recombinant 20.8-kDa protein of Mycobacterium avium subsp. paratuberculosis-based sero-diagnosis of paratuberculosis. 3 Biotech. 2017;7(3):156doi: 10.1007/s13205-017-0758-x.
Goswami, P. P., Chand, G., Prasad, N. S., Deb, R., & Basagoudanavar, S. H. (2017). Recombinant 20.8-kDa protein of Mycobacterium avium subsp. paratuberculosis-based sero-diagnosis of paratuberculosis. 3 Biotech, 7(3), 156. https://doi.org/10.1007/s13205-017-0758-x
Goswami, P P, et al. "Recombinant 20.8-kDa protein of Mycobacterium avium subsp. paratuberculosis-based sero-diagnosis of paratuberculosis." 3 Biotech vol. 7,3 (2017): 156. doi: https://doi.org/10.1007/s13205-017-0758-x
Goswami PP, Chand G, Prasad NS, Deb R, Basagoudanavar SH. Recombinant 20.8-kDa protein of Mycobacterium avium subsp. paratuberculosis-based sero-diagnosis of paratuberculosis. 3 Biotech. 2017 Jul;7(3):156. doi: 10.1007/s13205-017-0758-x. Epub 2017 Jun 16. PMID: 28623492; PMCID: PMC5473781.
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3 Biotech. 2017 Jul;7(3):156. doi: 10.1007/s13205-017-0758-x. Epub 2017 Jun 16.

Recombinant 20.8-kDa protein of Mycobacterium avium subsp. paratuberculosis-based sero-diagnosis of paratuberculosis.

3 Biotech

P P Goswami, Gokul Chand, N S Prasad, R Deb, S H Basagoudanavar

Affiliations

  1. Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar, 243122, India. [email protected].
  2. Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar, 243122, India.

PMID: 28623492 PMCID: PMC5473781 DOI: 10.1007/s13205-017-0758-x

Abstract

Johne's disease or paratuberculosis is a chronic infectious enteric disease of ruminants caused by the intracellular pathogen. The control of the Johne's disease is hampered by lack of specific diagnostic tests. In this study, we have cloned and expressed the N-terminal region of the locus tag Map 1637c encoding 20.8-kDa (r20.8) protein of Mycobacterium avium subsp. paratuberculosis. The recombinant protein r20.8 was expressed in high levels in Escherichia coli. The protein r20.8 was purified by single-step chromatography using Ni-NTA agarose. The protein r20.8 was reacted with anti-r20.8 antibodies as well as cattle sera infected with Map on Western blot. ELISA using well-characterized sera (both positive and negative; n = 60 each) Map-infected and non-infected cattle, respectively, yielded a sensitivity of 73.3% and a specificity of 98.3%. The 20.8 kDa protein expressed in the present study will prove useful as reagent in diagnostic test.

Keywords: 20.8 kDa; Mycobacterium avium subsp. paratuberculosis

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