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Mira A, Artacho A, Camelo-Castillo A, et al. Salivary Immune and Metabolic Marker Analysis (SIMMA): A Diagnostic Test to Predict Caries Risk. Diagnostics (Basel). 2017;7(3)doi: 10.3390/diagnostics7030038.
Mira, A., Artacho, A., Camelo-Castillo, A., Garcia-Esteban, S., & Simon-Soro, A. (2017). Salivary Immune and Metabolic Marker Analysis (SIMMA): A Diagnostic Test to Predict Caries Risk. Diagnostics (Basel, Switzerland), 7(3), . https://doi.org/10.3390/diagnostics7030038
Mira, Alex, et al. "Salivary Immune and Metabolic Marker Analysis (SIMMA): A Diagnostic Test to Predict Caries Risk." Diagnostics (Basel, Switzerland) vol. 7,3 (2017). doi: https://doi.org/10.3390/diagnostics7030038
Mira A, Artacho A, Camelo-Castillo A, Garcia-Esteban S, Simon-Soro A. Salivary Immune and Metabolic Marker Analysis (SIMMA): A Diagnostic Test to Predict Caries Risk. Diagnostics (Basel). 2017 Jun 27;7(3). doi: 10.3390/diagnostics7030038. PMID: 28654016; PMCID: PMC5617938.
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Diagnostics (Basel). 2017 Jun 27;7(3). doi: 10.3390/diagnostics7030038.

Salivary Immune and Metabolic Marker Analysis (SIMMA): A Diagnostic Test to Predict Caries Risk.

Diagnostics (Basel, Switzerland)

Alex Mira, Alejandro Artacho, Anny Camelo-Castillo, Sandra Garcia-Esteban, Aurea Simon-Soro

Affiliations

  1. Center for Advanced Research in Public Health, FISABIO Foundation, Valencia 46020, Spain. [email protected].
  2. Center for Advanced Research in Public Health, FISABIO Foundation, Valencia 46020, Spain. [email protected].
  3. Center for Advanced Research in Public Health, FISABIO Foundation, Valencia 46020, Spain. [email protected].
  4. Center for Advanced Research in Public Health, FISABIO Foundation, Valencia 46020, Spain. [email protected].
  5. Center for Advanced Research in Public Health, FISABIO Foundation, Valencia 46020, Spain. [email protected].

PMID: 28654016 PMCID: PMC5617938 DOI: 10.3390/diagnostics7030038

Abstract

By using ELISA and colorimetric tests, we have measured 25 compounds in individuals with and without dental caries at different time points of dental biofilm formation and time of the day. We find that some compounds appear to be affected by circadian rhythms, others by dental plaque maturity, and others show constant values during a 24 h period. Using univariate analysis and cross-validation techniques, we have selected six components measured at specific time points that maximize the diagnostic separation of health and disease conditions. Two out of the six selected compounds are related to immune competence, another two to the adhesion capacity of micro-organisms, and another two to acid production or pH buffering. We conclude that, in order to design a robust caries risk test, the time of saliva sampling must be standardized and biomarkers from different categories must be included. The preliminary data shown in this paper provide a proof of principle of a caries risk test based on risk-associated categories. Thus, the test will provide not only a general caries risk assessment, but also the likely biological origin of that risk, namely: immune imbalance, and/or a tendency to adhesion of cariogenic organisms, and/or a lack of acid buffering. When tested longitudinally and validated in larger cohorts, this could open the possibility to develop preventive and personalized treatments.

Keywords: adhesion; buffering capacity; circadian rhythms; dental caries; immune system; microorganisms; pH; saliva; toothpaste

Conflict of interest statement

The authors declare no conflict of interest. The public founding sponsors had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscri

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