Display options
Cite
Donnelly H, Smith CA, Sweeten PE, et al. Bone and cartilage differentiation of a single stem cell population driven by material interface. J Tissue Eng. 2017;8:2041731417705615doi: 10.1177/2041731417705615.
Donnelly, H., Smith, C. A., Sweeten, P. E., Gadegaard, N., Meek, R. D., D'Este, M., Mata, A., Eglin, D., & Dalby, M. J. (2017). Bone and cartilage differentiation of a single stem cell population driven by material interface. Journal of tissue engineering, 82041731417705615. https://doi.org/10.1177/2041731417705615
Donnelly, Hannah, et al. "Bone and cartilage differentiation of a single stem cell population driven by material interface." Journal of tissue engineering vol. 8 (2017): 2041731417705615. doi: https://doi.org/10.1177/2041731417705615
Donnelly H, Smith CA, Sweeten PE, Gadegaard N, Meek RD, D'Este M, Mata A, Eglin D, Dalby MJ. Bone and cartilage differentiation of a single stem cell population driven by material interface. J Tissue Eng. 2017 May 15;8:2041731417705615. doi: 10.1177/2041731417705615. eCollection 2017. PMID: 28567273; PMCID: PMC5438107.
Copy Download .nbib Format: NLM
Share it on

J Tissue Eng. 2017 May 15;8:2041731417705615. doi: 10.1177/2041731417705615. eCollection 2017.

Bone and cartilage differentiation of a single stem cell population driven by material interface.

Journal of tissue engineering

Hannah Donnelly, Carol-Anne Smith, Paula E Sweeten, Nikolaj Gadegaard, Rm Dominic Meek, Matteo D'Este, Alvaro Mata, David Eglin, Matthew J Dalby

Affiliations

  1. Centre for Cell Engineering, University of Glasgow, Glasgow, UK.
  2. Division of Biomedical Engineering, University of Glasgow, Glasgow, UK.
  3. Department of Orthopaedics, Southern General Hospital, Glasgow, UK.
  4. AO Research Institute Davos, Davos, Switzerland.
  5. Institute of Bioengineering, Queen Mary University of London, London, UK.
  6. School of Engineering and Materials Science, Queen Mary University of London, London, UK.

PMID: 28567273 PMCID: PMC5438107 DOI: 10.1177/2041731417705615

Abstract

Adult stem cells, such as mesenchymal stem cells, are a multipotent cell source able to differentiate towards multiple cell types. While used widely in tissue engineering and biomaterials research, they present inherent donor variability and functionalities. In addition, their potential to form multiple tissues is rarely exploited. Here, we combine an osteogenic nanotopography and a chondrogenic hyaluronan hydrogel with the hypothesis that we can make a complex tissue from a single multipotent cell source with the exemplar of creating a three-dimensional bone-cartilage boundary environment. Marrow stromal cells were seeded onto the topographical surface and the temperature gelling hydrogel laid on top. Cells that remained on the nanotopography spread and formed osteoblast-like cells, while those that were seeded into or migrated into the gel remained rounded and expressed chondrogenic markers. This novel, simple interfacial environment provides a platform for anisotropic differentiation of cells from a single source, which could ultimately be exploited to sort osteogenic and chondrogenic progenitor cells from a marrow stromal cell population and to develop a tissue engineered interface.

Keywords: Nanotopography; complex tissue engineering; endochondral tissue engineering; hydrogel; mesenchymal stem cells

Conflict of interest statement

Declaration of conflicting interests: The author(s) declared no potential conflicts of interest with respect to the research, authorship and/or publication of this article.

References

  1. Biomaterials. 2013 Mar;34(9):2177-84 - PubMed
  2. Acta Biomater. 2014 Feb;10(2):651-60 - PubMed
  3. J Cell Biochem. 2014 Feb;115(2):380-90 - PubMed
  4. Soft Matter. 2009;5(6):1228-1236 - PubMed
  5. Nat Mater. 2007 Dec;6(12):997-1003 - PubMed
  6. Methods. 2001 Dec;25(4):402-8 - PubMed
  7. Int J Exp Pathol. 2012 Dec;93(6):389-400 - PubMed
  8. J Bone Miner Res. 2003 Jan;18(1):47-57 - PubMed
  9. J Cell Sci. 2004 Jan 26;117(Pt 3):417-25 - PubMed
  10. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2005 Aug;19(8):652-7 - PubMed
  11. ACS Nano. 2014 Oct 28;8(10):9941-53 - PubMed
  12. J Neurosci. 2008 Dec 17;28(51):13978-84 - PubMed
  13. Biomaterials. 2011 Jul;32(21):4793-805 - PubMed
  14. Biomaterials. 2011 May;32(13):3413-22 - PubMed
  15. Stem Cells Dev. 2011 Jun;20(6):1099-112 - PubMed
  16. J Cell Biol. 2007 Feb 26;176(5):709-18 - PubMed
  17. Osteoarthritis Cartilage. 2011 Feb;19(2):222-32 - PubMed
  18. ACS Nano. 2012 Nov 27;6(11):10239-49 - PubMed
  19. Nat Mater. 2011 Jul 17;10(8):637-44 - PubMed
  20. Spine J. 2013 Nov;13(11):1627-39 - PubMed
  21. Biomaterials. 2009 Sep;30(27):4610-7 - PubMed
  22. Sci Adv. 2016 Aug 26;2(8):e1600188 - PubMed
  23. J Biomed Mater Res A. 2016 Jun;104(6):1469-78 - PubMed
  24. Dev Growth Differ. 2011 Apr;53(3):357-65 - PubMed
  25. Carbohydr Polym. 2012 Oct 15;90(3):1378-85 - PubMed
  26. Dev Biol. 2002 May 1;245(1):95-108 - PubMed
  27. Adv Mater. 2010 Jan 12;22(2):175-89 - PubMed
  28. Acta Biomater. 2014 Oct;10(10):4340-50 - PubMed
  29. Cell. 1997 Feb 7;88(3):287-98 - PubMed
  30. Cell. 2006 Aug 25;126(4):677-89 - PubMed
  31. ACS Nano. 2013 Mar 26;7(3):2758-67 - PubMed
  32. Nat Mater. 2008 Oct;7(10):816-23 - PubMed
  33. Acta Biomater. 2012 Sep;8(9):3283-93 - PubMed
  34. Proc Natl Acad Sci U S A. 2010 Mar 16;107(11):4872-7 - PubMed
  35. J Cell Biochem. 2007 Feb 1;100(2):326-38 - PubMed

Publication Types