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Sahay H, Yadav AN, Singh AK, et al. Hot springs of Indian Himalayas: potential sources of microbial diversity and thermostable hydrolytic enzymes. 3 Biotech. 2017;7(2):118doi: 10.1007/s13205-017-0762-1.
Sahay, H., Yadav, A. N., Singh, A. K., Singh, S., Kaushik, R., & Saxena, A. K. (2017). Hot springs of Indian Himalayas: potential sources of microbial diversity and thermostable hydrolytic enzymes. 3 Biotech, 7(2), 118. https://doi.org/10.1007/s13205-017-0762-1
Sahay, Harmesh, et al. "Hot springs of Indian Himalayas: potential sources of microbial diversity and thermostable hydrolytic enzymes." 3 Biotech vol. 7,2 (2017): 118. doi: https://doi.org/10.1007/s13205-017-0762-1
Sahay H, Yadav AN, Singh AK, Singh S, Kaushik R, Saxena AK. Hot springs of Indian Himalayas: potential sources of microbial diversity and thermostable hydrolytic enzymes. 3 Biotech. 2017 Jun;7(2):118. doi: 10.1007/s13205-017-0762-1. Epub 2017 May 31. PMID: 28567630; PMCID: PMC5451362.
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3 Biotech. 2017 Jun;7(2):118. doi: 10.1007/s13205-017-0762-1. Epub 2017 May 31.

Hot springs of Indian Himalayas: potential sources of microbial diversity and thermostable hydrolytic enzymes.

3 Biotech

Harmesh Sahay, Ajar Nath Yadav, Atul Kumar Singh, Surendra Singh, Rajeev Kaushik, Anil Kumar Saxena

Affiliations

  1. Department of Biological Science, Rani Durgavati University, Jabalpur, India.
  2. Department of Research and Development, R-Biopharm Neugen Group, Hyderabad, India.
  3. Department of Biotechnology, Akal College of Agriculture, Eternal University, Baru Sahib, India.
  4. Division of Infectious Diseases, Boston Children's Hospital, Harvard Medical School, Boston, MA, USA.
  5. Division of Microbiology, Indian Agricultural Research Institute, New Delhi, India.
  6. National Bureau of Agriculturally Important Microorganisms, Kushmaur, Mau Nath Bhanjan, Mau, Uttar Pradesh, 275103, India. [email protected].

PMID: 28567630 PMCID: PMC5451362 DOI: 10.1007/s13205-017-0762-1

Abstract

Microbial communities in hot springs at high elevations have been extensively studied worldwide. In this sense, the Indian Himalaya regions is valuable ecosystems for providing both the extreme 'cold' and 'hot' sites for exploring microbial diversity. In the present study, a total of 140 thermophilic bacteria were isolated from 12 samples collected from Manikaran and Yumthang hot springs of Indian Himalayas. The bacterial isolates were studied for phylogenetic profiling, growth properties at varying conditions and potential sources of extracellular thermostable hydrolytic enzymes such as protease, amylase, xylanase and cellulase. Based on production of extracellular hydrolases, 51 isolates from Manikaran (28) and Yumthang thermal springs (23) were selected and identified using 16S rRNA gene sequencing which included 37 distinct species of 14 different genera namely Anoxybacillus, Bacillus, Brevibacillus, Brevundimonas, Burkholderia, Geobacillus, Paenibacillus, Planococcus, Pseudomonas, Rhodanobacter, Thermoactinomyces, Thermobacillus, Thermonema and Thiobacillus. Out of 51 hydrolase producing bacteria, 24 isolates showed stability at wide range of temperature and pH treatments. In present investigation, three thermotolerant bacteria namely, Thermobacillus sp NBM6, Paenibacillus ehimensis NBM24 and Paenibacillus popilliae NBM68 were found to produced cellulase-free xylanase. These potential extracellular thermostable hydrolytic enzymes producing thermophilic bacteria have a great commercial prospect in various industrial, medical and agriculture applications.

Keywords: 16S rRNA gene; Diversity; Himalayan hot springs; Thermophiles; Thermostable extracellular hydrolases

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