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Shi QQ, Dang J, Wen HX, et al. Anti-hepatitis, antioxidant activities and bioactive compounds of Dracocephalum heterophyllum extracts. Bot Stud. 2016;57(1):16doi: 10.1186/s40529-016-0133-y.
Shi, Q. Q., Dang, J., Wen, H. X., Yuan, X., Tao, Y. D., & Wang, Q. L. (2016). Anti-hepatitis, antioxidant activities and bioactive compounds of Dracocephalum heterophyllum extracts. Botanical studies, 57(1), 16. https://doi.org/10.1186/s40529-016-0133-y
Shi, Qiang-Qiang, et al. "Anti-hepatitis, antioxidant activities and bioactive compounds of Dracocephalum heterophyllum extracts." Botanical studies vol. 57,1 (2016): 16. doi: https://doi.org/10.1186/s40529-016-0133-y
Shi QQ, Dang J, Wen HX, Yuan X, Tao YD, Wang QL. Anti-hepatitis, antioxidant activities and bioactive compounds of Dracocephalum heterophyllum extracts. Bot Stud. 2016 Dec;57(1):16. doi: 10.1186/s40529-016-0133-y. Epub 2016 Aug 06. PMID: 28597426; PMCID: PMC5432916.
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Bot Stud. 2016 Dec;57(1):16. doi: 10.1186/s40529-016-0133-y. Epub 2016 Aug 06.

Anti-hepatitis, antioxidant activities and bioactive compounds of Dracocephalum heterophyllum extracts.

Botanical studies

Qiang-Qiang Shi, Jun Dang, Huai-Xiu Wen, Xiang Yuan, Yan-Duo Tao, Qi-Lan Wang

Affiliations

  1. Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, CAS, Xining, China.
  2. University of Chinese Academy of Science, Beijing, China.
  3. Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, CAS, Xining, China. [email protected].

PMID: 28597426 PMCID: PMC5432916 DOI: 10.1186/s40529-016-0133-y

Abstract

BACKGROUND: Dracocephalum heterophyllum was a traditional Tibetan medicine possesses various pharmacological effects involved in anti-inflammatory, antibacterial activities. However, its anti-hepatitis, antioxidant activity and bioactive compounds have not been reported, the objective of this research work was to investigate the pharmacological activity and bioactive compounds of D. heterophyllum extracts.

RESULTS: In the present study, the anti-hepatics and antioxidant activities of four D. heterophyllum extracts (i.e. petroleum ether extracts, ethyl acetate extracts, n-BuOH extracts, and water extracts) were conducted. The main chemical constituent of petroleum ether and ethyl acetate extracts were also isolated using chromatographic techniques and identified by NMR spectroscopic methods. The anti-hepatitis assay showed that the petroleum ether and ethyl acetate extracts of D. heterophyllum significantly prolonged the mean survival times and reduced the mortality of mouse hepatitis model induced by concanavalin A (ConA). The levels of alanine transaminase, aspartate transaminase in blood serum could be decreased obviously by ethyl acetate extracts compared with ConA group (P < 0.01). The histological analysis demonstrated that the ethyl acetate extracts could inhibit apoptosis and necrosis caused by ConA. In addition, the antioxidant activities of the four extracts of D. heterophyllum were measured by DPPH assay, ABTS assay, anti-lipidperoxidation assay, ferric reducing antioxidant power assay, ferrous metal ions chelating assay and determination of total phenolic contents. The results showed that the ethyl acetate extract had the highest antioxidant activities, followed by petroleum ether extract. Finally, nine mainly compounds were isolated from the Petroleum ether and ethyl acetate extracts, including four triterpenes: oleanolic acid (1), ursolic acid (2), pomolic acid (3), 2α- hydroxyl ursolic acid (4), three flavonoids: apigenin-7-O-rutinoside (5), luteolin (8), diosmetin (9) and two phenolic acids: rosmarinic acid (6), methyl rosmarinate (7).

CONCLUSION: The Ethyl acetate extract of D. heterophyllum had the highest anti-hepatitis and antioxidants activities, followed by petroleum ether extract. The bioactive substances may be triterpenes, flavonoids and phenolic acids, the ethyl acetate extracts of D. heterophyllum may be possible candidates in developing anti-hepatitis medicine.

Keywords: Anti-hepatitis activity; Antioxidant activity; Chemical constituents; Dracocephalum heterophyllum

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